邻苯二甲酸二异辛酯致赤子爱胜蚓体细胞氧化损伤的研究  

Oxidation Damage Induced by Di-(2-ethylhexyl)Phthlate on the Cells of Esisenia foelide

作　　者：曾强[1] 程文文[1] 蔡凤云[1] 胡传禄[1] 王婧[1] 杨旭[1] 

机构地区：[1]华中师范大学生命科学学院环境科学实验室和湖北省遗传调控与整合生物学重点实验室,武汉430079

出　　处：《生态毒理学报》2010年第5期718-723,共6页Asian Journal of Ecotoxicology

基　　金：国家自然科学基金面上项目(No.51076079)

摘　　要：以赤子爱胜蚓为实验材料,探讨了邻苯二甲酸二异辛酯(DEHP)对赤子爱胜蚓(Esisenia foelide)的毒性.DEHP染毒剂量:0、0.2、0.4、0.8、1.6mmol·L-1,染毒2h后采用KCl-SDS沉淀法检测细胞匀浆DNA-蛋白质交联(DPC)系数,硫代巴比妥酸法检测丙二醛(MDA)含量.结果表明:随着DEHP染毒浓度的增加,赤子爱胜蚓细胞匀浆的DPC系数显著升高(F=14.1,p<0.01),并具有剂量-效应关系(r=0.8877,p<0.05),而MDA含量变化不显著,各染毒组与对照组之间不存在显著性差异(F=0.27,p>0.05).以上结果表明,实验浓度的DEHP暴露可使赤子爱胜蚓体细胞发生显著的DNA蛋白质交联,具有遗传毒性.This study took Esisenia foelide as experimental material to explore the soil-ecotoxicity induced by di-（2-ethylhexyl）phthalate（DEHP）.The exposure doses were 0,0.2,0.4,0.8,1.6mmol·L-1.The Esisenia foelide were exposed to DEHP for 2 hours in vivo with the bath exposure technique.KCl-SDS assay and thiobarbituric acid assay were used to measure the change of the coefficient of DNA-protein crosslinks（DPC）and the contents of malondialdehyde（MDA）of somatic cells,respectively.Results showed that DPC coefficient increased significantly with the increase of DEHP concentration（F=14.1,p0.01）,and showed a dose-effect manner relationship（r=0.8877,p0.05）,but the contents of MDA did not change significantly with the increase of DEHP concentrations（F=0.27,p0.05）.Above results indicated that DEHP exposure could induce significant DNA-protein crosslinks of somatic cells of Esisenia foelid.

关 键 词：邻苯二甲酸二异辛酯 赤子爱胜蚓 DNA-蛋白质交联 丙二醛 

分 类 号：X171.5[环境科学与工程—环境科学]