三种典型纳米颗粒物造成的人体肺细胞氧化应激和炎症效应  

Oxidative Stress and Inflammatory Effect on A549 Cell Line Induced by Three Typical Nano-Particles

作　　者：李可欣[1] 曹红英[1] 王斌[2] 张羽中[2] 史科路[2] 陶澍[2] 

机构地区：[1]中科院地理与资源研究所,北京100101 [2]北京大学城市与环境学院地表过程分析与模拟教育部重点实验室,北京100871

出　　处：《生态毒理学报》2010年第6期787-792,共6页Asian Journal of Ecotoxicology

基　　金：国家自然科学基金(No.40503018;No.40771179);国家环保部公益项目(No.200809101)

摘　　要：采用体外细胞暴露实验研究了人肺腺癌细胞系(A549)单层细胞暴露于50和500μg·mL-1两种浓度纳米氧化钛、纳米氧化硅、碳纳米管和晶体石英砂等四种颗粒物后产生的氧化应激和炎症反应.用细胞活度、细胞内活性氧总量和细胞上清液中白细胞介素8(IL-8)表达量表征暴露效应.研究结果表明,纳米氧化钛、纳米氧化硅和碳纳米管在体外暴露实验过程中均发生不同程度的聚集;细胞暴露48h后,三种纳米颗粒物均使A549细胞活度下降,诱导细胞产生过量活性氧,同时刺激细胞IL-8表达量增高;三种纳米颗粒物中,纳米氧化钛和纳米氧化硅对细胞活度影响较大,碳纳米管诱发的炎症效应较另两种纳米材料强.In this study,oxidative damage and inflammatory effect on A549 cells induced by four typical particles including crystalline silica（CS）,nanotitanium dioxide（nano-TiO2）,nanosilicon titanium dioxide（nano-SiO2）,and multi-walled carbon nanotube（MWCT）have been investigated using an in-vitro method.The A549 cell monolayer was exposed to the nano-particles at 50 and 500μg·mL-1 for 48h.MTT assay was performed to evaluate the viability of A549 cells after 48h exposure.The level of reactive oxygen species（ROS）in the cells and interleukin-8（IL-8）in the cell culture medim supernatant liquid were also measured.The results revealed that in comparison with CS,nano-TiO2,nano-SiO2,and MWCT had higher potential for inducing ROS generation.Nano-TiO2,nano-SiO2,and MWCT demonstrated similar effects on A549 cell viability as CS.No significant difference in MTT was detected among MWCT,nano-TiO2,and nano-SiO2.The three nano-particles can induce both oxidative stress and inflammatory effect on A549 cells.

关 键 词：纳米颗粒 体外暴露 氧化性损伤 炎症效应 A549细胞株 

分 类 号：X171.5[环境科学与工程—环境科学]