有机磷细菌的分离鉴定及其对MC-RR的响应  

The Isolation and Identification of Organic Phosphorus Bacteria and Its Response to MC-RR

作　　者：杨翠云[1] 刘永定[2] 夏传海[1] 刘苏静[1] 周世伟[1] 

机构地区：[1]中国科学院烟台海岸带研究所,烟台264003 [2]中国科学院水生生物研究所,武汉430072

出　　处：《生态毒理学报》2010年第6期829-834,共6页Asian Journal of Ecotoxicology

基　　金：国家海洋局重点实验室开放基金(No.200713);国家海藻工程中心开放基金(No.OK0810BX-078);中科院知识创新工程方向性项目(No.KZCX2-YW-Q07-04)

摘　　要：从云南滇池水样中分离出具有解磷能力的有机磷细菌P-2,并利用现代分子生物学技术进行了初步鉴定.用0.01、5mg·L-1微囊藻毒素(MC-RR)处理有机磷细菌P-2,研究了MC-RR对其生长、细胞内酸碱磷酸酶活性(ACP和AKP)以及培养液中可溶性磷酸盐含量的影响.结果表明,高浓度MC-RR能显著抑制有机磷细菌的生长,延缓其细胞增殖,抑制细胞内酸碱磷酸酶活性以及培养液中可溶性磷酸盐含量的升高,因而可能改变或减缓生态系统中磷循环的进程,这表明微囊藻毒素在一定程度上可能调节水体细菌功能群落.The organic phosphorus bacteria was isolated from Dianchi Lake in Yunnan,and identified by the modern molecular biology techniques.The effect of MC-RR on the growth and physiological characteristics including acid/alkaline phosphatase（ACP/AKP） activity and soluble phosphate contents were studied by using 0.01,5mg·L-1 MC-RR to treat organic phosphorus bacteria.The results show that MC-RR with high concentration could significantly inhibit the growth of organic phosphorus bacteria,delay its cell proliferation,inhibit ACP and AKP activity in cells and increase soluble phosphate contents in the liquid culture medium,which imply the process of phosphorus cycle might be inhibited or slowed down in ecosystem and MC-RR might play an important role on regulating bacterial functional communities in some aquatic environments.

关 键 词：微囊藻毒素(MC-RR) 生长 酸碱磷酸酶 可溶性磷酸盐 

分 类 号：X172[环境科学与工程—环境科学] X171.5