不同生育期灵芝子实体水提物与其免疫活性  

Effect of Aqueous Extracts from Ganoderma Fruit Bodies Harvested at Different Stages of Development on Nitric Oxide Production by RAW 264.7 Cells

作　　者：冯娜[1] 张劲松[1] 唐传红[1] 刘艳芳[1] 郝瑞霞[2] 杨焱[1] 唐庆九[1] 贾薇[1] 汪文瀚[1] 吴迪[1] 周帅[1] 刘方[1] 

机构地区：[1]国家食用菌工程技术研究中心,农业部应用真菌资源与利用重点开放实验室,上海市农业遗传育种重点开放实验室,上海市农业科学院食用菌研究所,上海201106 [2]鄂尔多斯市疾病预防控制中心,内蒙古鄂尔多斯017000

出　　处：《食用菌学报》2010年第4期52-57,共6页Acta Edulis Fungi

基　　金：上海市农业科学院科技发展基金[编号:上海市农业科学院农科发2008(15)]的部分研究内容

摘　　要：分析4个灵芝(Ganoderma)菌株不同生育期子实体水提物的提取得率、多糖含量、多糖分子量分布和其对巨噬细胞RAW 264.7 NO释放量的影响。结果表明,随着灵芝的生长发育,其子实体水提物得率呈逐渐降低的趋势,水提物中多糖含量差异显著。水提物经透析后其多糖的分子量分布主要集中在12 kDa至25 kDa之间,但不同生育期间的各分子量多糖的含量存在差异。芝蕾发育初期和成熟后期子实体水提物透析后的免疫活性较好。综合考量,认为成熟后期采收的灵芝子实体虽然多糖含量较低,但生物量较大,可获得较大量活性多糖,所以确定成熟后期为适宜采收期。Fruit bodies of four Ganoderma strains were collected at five different stages of development,dried to constant weight at 60 ℃,ground into a powder and extracted with CHCl3 at room temperature for 6 h.The dried residue material was further extracted with boiling water for 1 h and the percentage extraction rate（defined as mg dry weight of extract divided by mg dry weight of starting material×100）,the content and molecular weight distribution of polysaccharides in the extract,and the effects on NO production by RAW 264.7 cells,were determined.Extraction rates gradually decreased with fruiting body development,and the polysaccharide content of the extracts varied significantly according to the developmental stage.After dialysis,the molecular weights of the polysaccharides varied between 12 kDa and 25 kDa,and the amounts of polysaccharide with different molecular weights varied according to the developmental stage.Immunological activity of dialysed aqueous extracts was highest at the bud forming stage,and was also strong in extracts of post-maturation material.We conclude that,in view of the strong immunological activity of the latter and that the mature fruit bodies have a considerably greater biomass compared with the buds,the post-mature fruit bodies represented the optimal harvest time even though their polysaccharide content was relatively low.

关 键 词：灵芝 子实体采收期 水提物 多糖含量 分子量分布 NO释放量 

分 类 号：S567.31[农业科学—中草药栽培]