Determination of metoprolol in rabbit blood using capillary electrophoresis with laser-induced fluorescence detection  

作　　者：Yu Yun Chen Wei Ping Yang Zhu Jun Zhang 

机构地区：[1]College of Environmental Science and Engineering, Chang'an University, Xi'an 710054, China [2]Education School of Nationalities, Shaanxi Normal University, Xi'an 710062, China [3]Department of Chemistry, Shaanxi Normal University, Xi'an 710062, China

出　　处：《Chinese Chemical Letters》2011年第3期350-353,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No30470886)

摘　　要：This work described a sensitive method for determination of metoprolol in rabbit plasma.The method involved purification by ultrafiltration,derivatization with fluorescein isothiocyanate,determination by capillary electrophoresis（CE） coupled with laser-induced fluorescence（LIF） detector.Other components in plasma including a variety of amino acids and proteins did not interfere with the determination of metoprolol in experimental condition.The assay had a wide range（2.0-500 ng/mL） of linearity and a detection limit of 0.8 ng/mL.The intra- and inter-day precisions were satisfactory with relative standard deviation（RSD） less than 10.0%and accuracy within 10.0%.This method was successfully applied to pharmacokinetic study of metoprolol in rabbit blood.This work described a sensitive method for determination of metoprolol in rabbit plasma.The method involved purification by ultrafiltration,derivatization with fluorescein isothiocyanate,determination by capillary electrophoresis（CE） coupled with laser-induced fluorescence（LIF） detector.Other components in plasma including a variety of amino acids and proteins did not interfere with the determination of metoprolol in experimental condition.The assay had a wide range（2.0-500 ng/mL） of linearity and a detection limit of 0.8 ng/mL.The intra- and inter-day precisions were satisfactory with relative standard deviation（RSD） less than 10.0%and accuracy within 10.0%.This method was successfully applied to pharmacokinetic study of metoprolol in rabbit blood.

关 键 词：Capillary electrophoresis METOPROLOL Laser-induced fluorescence ULTRAFILTRATION PHARMACOKINETIC 

分 类 号：O657.8[理学—分析化学] TH744.4[理学—化学]