脱氧雪腐镰刀菌烯醇对BHK-21细胞的线粒体膜电位及Bax和Bcl-2蛋白表达的影响  

Effect of deoxynivalenol on the mitochondrial membrane potentials and Bax,Bcl-2 expression of BHK-21 cells

作　　者：丁举静[1] 何成华[1] 刘俐君 马彦娜[1] 张海彬[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]南京日升昌康华药业有限公司,江苏南京210007

出　　处：《南京农业大学学报》2011年第2期101-106,共6页Journal of Nanjing Agricultural University

基　　金：国家863计划项目(2007AA10Z429); 国家自然科学基金项目(30771595;30901091); 江苏省农业科技自主创新基金项目(CX(08)135)

摘　　要：为了探讨脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)对体外培养仓鼠肾细胞(BHK-21)的线粒体膜电位及Bax和Bcl-2蛋白表达的影响,采用细胞培养、流式细胞术、免疫细胞化学染色等方法,研究DON对细胞早期凋亡率、线粒体膜电位及Bax和Bcl-2蛋白表达的变化。结果显示:不同浓度的DON均可诱导BHK-21细胞凋亡,各浓度组的凋亡率都显著高于对照组(P<0.05);DON可使BHK-21细胞线粒体膜电位下降,且表现出剂量和时间效应关系。免疫组织化学染色显示,DON处理细胞24 h,Bax蛋白表达显著高于对照组,而Bcl-2蛋白表达均低于对照组。上述结果表明:DON诱导了BHK-21细胞凋亡和线粒体膜电位下降,Bax表达增强而Bcl-2表达下降是DON诱导BHK-21细胞凋亡的分子机制之一。To investigate the effect of deoxynivalenol（DON）on the mitochondrial membrane potentials（MMP）and Bax,Bcl-2 expression of hamster kidney cells（BHK-21 cell）in vitro,the change of early apoptosis,mitochondrial membrane potential and apoptosis-related proteins with Bax and Bcl-2 expression were measured by flow cytometry and immunocytochemical staining assay.The results showed that different concentration of DON could induce apoptosis for BHK-21 cells within the experimental concentration and cause apoptosis significantly as compared with control group（P〈0.05）.A marked decrease of mitochondrial membrane potential in a dose and time dependent manner were observed clearly by mitochondrial membrane potentials assay.Immunohistochemical staining analysis showed that,after 24 h treatment with DON,the expression of Bax was significantly higher,while Bcl-2 decreased.These result suggested that DON could induce apoptosis and the MMP disruptionin of BHK-21 cells in vitro.The essential mechanisms of apoptosis might be related to inducing the change of hepatocarcinoma cell cycle,the expression of the Bax increase and Bcl-2 decrease in BHK-21 cells.

关 键 词：脱氧雪腐镰刀菌烯醇 仓鼠肾细胞(BHK-21) 线粒体膜电位 细胞凋亡 BAX Bcl-2 

分 类 号：R992[医药卫生—毒理学]