家族性原发性高血压与血管紧张素原基因M 235T突变关系的研究  

The Association Between M235T Variant of Angiotensinogen Gene and Familial Essential Hypertension

作　　者：黄诚群[1] 张红叶[1] 丁金凤[2] 黄尚志[3] 李莹[1] 杨军[1] 公茂莲[1] 田秀珍 

机构地区：[1]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院流行病学研究室,北京市100037 [2]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院,北京市100037 [3]中国医学科学院中国协和医科大学基础医学研究所医学遗传中心 [4]北京市石景山区慢性病防治所

出　　处：《中国循环杂志》1999年第4期222-224,共3页Chinese Circulation Journal

基　　金：国家自然科学基金

摘　　要：目的：探讨血管紧张素原基因Ｍ２３５Ｔ突变与家族性原发性高血压（ＥＨ）的关系。　　方法：应用脱氧核糖核酸（ＤＮＡ）杂交和测序检定方法，对一个典型原发性高血压大家系１００名成员［分直系亲属高血压组（ｎ＝ ４５）、直系亲属正常血压组（ｎ＝ ３８）及非直系亲属正常血压组（ｎ＝ １７）］和正常血压对照家系直系亲属２１ 名（为正常对照组）成员按＜ ３５岁和≥３５岁２个年龄层的Ｍ２３５Ｔ突变进行分析。　　结果：比较４组在＜ ３５ 岁和≥３５ 岁２个年龄层的Ｍ２３５Ｔ分布。表明各年龄层中，４组间Ｍ２３５Ｔ基因型及基因频率分布未见显著不同。　　结论：血管紧张素原基因Ｍ２３５Ｔ突变与该家族性原发性高血压没有显著关联；Ｍ２３５Ｔ突变可能不是该家族性原发性高血压的遗传易感因素。Objective:To study the association between M235T variant of angiotensinogen gene (AGT) and familial essential hypertension(EH). Methods:Molecular hybridization and DNA sequencing were performed to detect the variant in all 121 subjects.There were 100 subjects in a large EH pedigree who were devided into 3 groups:direct relatives in EH ( n =45),direct relatives in normotensives( n =38),non direct relatives in normotensives( n =17).The other 21 subjects were normal controls. Results:The comparision of genotypes and gene frequency of M235T among hypertensive group and the other three normaltesive groups stratified by age at 35 years showed no significant difference in any groups. Conclusions:These results suggest that there is no significant relationship between M235T of AGT and the familial EH.M235T variant might not be a genetic susceptible factor for this familial EH.

关 键 词：高血压 遗传 血管紧张素原 M235T 基因突变 

分 类 号：R544.102[医药卫生—心血管疾病]