镉和汞两种重金属离子对四角蛤蜊血细胞DNA损伤的初步研究  

Effects of Cd^(2+) and Hg^(2+) on DNA damage in haemocytes of Mactra veneriformis

作　　者：房燕[1,2] 杨红生[1] 

机构地区：[1]中国科学院海洋研究所海洋生态与环境科学重点实验室,山东青岛266071 [2]中国科学院研究生院,北京100049

出　　处：《海洋科学》2011年第2期1-5,共5页Marine Sciences

基　　金：国家基金委创新研究群体科学基金项目(40821004);国家973计划资助项目(2007CB407305);国家农业部公益性计划资助项目(nyhyzx07-047);国家海洋公益性行业科研专项资助项目(200805069)

摘　　要：利用彗星电泳技术,研究了不同胁迫浓度和胁迫时间下,Cd2+和Hg2+对四角蛤蜊(Mactra veneriformis)血细胞DNA损伤的情况。研究结果显示,50、100和200μg/L Cd2+胁迫14天不能造成四角蛤蜊血细胞DNA损伤。10、20和40μg/L Hg2+胁迫均能明显损伤血细胞DNA,并且DNA损伤程度分别与Hg2+胁迫时间、胁迫浓度具有一定正相关性。200μg/L Cd2++20μg/L Hg2+复合胁迫组,四角蛤蜊血细胞DNA损伤程度随胁迫时间延长而增加,并且大于Hg2+胁迫组。上述结果表明对于四角蛤蜊而言,Hg2+潜在的遗传毒性大于Cd2+;Cd2+和Hg2+对DNA的损伤具有协同作用。The objective of this study was to investigate the effects of Cd2＋ and Hg2＋ on DNA damage in haemocytes of Mactra veneriformis that were exposed to different concentrations of Cd2＋ or/and Hg2＋ for 14 days.DNA damage was determined by the comet assay.There was no obvious DNA damage in haemocytes caused by 50,100,and 200 μg/L Cd2＋ during the exposure period.However,Hg2＋ caused significant DNA damage in haemocytes at doses of 10,20,and 40 μg/L in a dose-and time-dependent manner.Combination of 200μg/L Cd2＋ and 20μg/L Hg2＋ induced a time-dependent increase of DNA damage.The degree of DNA damage in the combined exposure group was severer than that in Hg2＋-treated groups.These results indicate that the ranking of genotoxic potential to M.veneriformis is in decreasing order： Hg2＋Cd2＋.There is a synergistic effect to DNA damage between Cd2＋ and Hg2＋.

关 键 词：四角蛤蜊(Mactra veneriformis) Cd2+ Hg2+ DNA损伤 彗星电泳 

分 类 号：X171.5[环境科学与工程—环境科学]