APE1基因沉默对人肝癌MHCC97-H生物学行为的影响  

Influences of APE1 gene silence on biological behavior of human hepatoma carcinoma cell line MHCC97-H

作　　者：杜薇[1] 李艳菊[1] 刘景丰[2] 郑智华[1] 高美钦[1] 黄爱民[1] 

机构地区：[1]福建医科大学基础医学院病理学系,福州350004 [2]福建医科大学附属第一医院肝病中心

出　　处：《中华实验外科杂志》2011年第4期557-560,共4页Chinese Journal of Experimental Surgery

基　　金：福建省教育厅科技资助项目，福建省教育厅基金资助项目

摘　　要：目的观察脱嘌吟／脱嘧啶核酸内切酶（APEl）基因表达沉默后对人高侵袭肝癌细胞株MHCC97-H在体内外生物学行为的影响。方法用LV-APEl-shRNA载体感染MHCC97-H，设空载体（LV—APEI—NC）组和空白对照（Contr01）组。通过噻唑蓝（MTT）比色法和Transwell小室法检测细胞的增殖、黏附、运动及侵袭能力的变化。建立裸鼠移植瘤动物模型，第21天剥瘤称重，计算抑瘤率，绘制肿瘤生长曲线。逆转录．聚合酶链反应（RT-PCR）及Westernblot法检测移植瘤中APElmRNA及蛋白表达水平。结果LV．APEl-shRNA组细胞的增殖、黏附、运动及侵袭能力均低于其他两组，差异有统计学意义（P〈0．01）。LV-APEl-shRNA组肿瘤体积（0．467±O．052）cm3及重量（0．267±0．082）g均小于LV—APEl-NC组（1．700±0．063）cm3（1．018±0．160）g和对照组（1．780±0．095）cm3、（1．107±0．178）g，差异有统计学意义（P〈0．01）；抑瘤率为75．881％。APEl基因沉默后可下调90．622％APElmRNA的表达，下调72．439％APEl蛋白的表达。结论APEl基因沉默可降低MHCC97-H细胞在体内外的增殖和侵袭能力。APEl对肝癌细胞的生物学行为具有调控作用。Objective To investigate the influences of apurinie/apyrimidinie endonuclease （ APE1 ） gene silence on malignant behaviors in vitro-in vivo of human hepatoma carcinoma cell line MH- CC97-H with high metastasis potential. Methods Three groups were set up： LV-APEI-shRNA transfected group, LV-APE1-NC empty vector transfected group and non-transfected group （control group）. The abili- ty of proliferation, adhesion, motility and invasion in vitrowas measured by using MTT assay and Transwell chamber. An animal model of the human hepatoma cancer xenograft was established. The intact subcutane- ous tumor tissues were obtained and their volumes and weight were measured. Tumor growth inhibition ratio was calculated. Reverse transcription-polymerase chain reaction （RT-PCR） assay and Western blotting were performed to detect the expression level of APE1. Results The ability of proliferation, adhesion, motility and invasion in LV-APE1-shRNA transfected group was lower as compared with other two groups （P 〈0. 01）. In LV-APEI-shRNA transfected group, the subcutaneous tumors grew slowly, the volume （0. 467 ±0. 052） cm3 and weight （0. 267 ±0. 082） g were reduced （P 〈0. 01）. The tumor growth inhibition ratio was 75. 881%. The expression levels of APE1 mRNA was decreased by 90. 584% and those of protein by 72. 439%. Conclusion APE1 gene silence of MHCC97-H can inhibit the ability of proliferation and in- vasion in vitro-in vivo. APE1 gene has effect on the tumor malignant behaviors of hepatoma carcinoma cells.

关 键 词：癌 肝细胞 APE1 裸鼠 侵袭 

分 类 号：R735.7[医药卫生—肿瘤]