参麦注射液对心肌缺血再灌注损伤大鼠ICAM-1表达的影响  

EFFECT OF SHENMAI INJECTION ON THE EXPRESSION OF ICAM-1 OF MYOCARDIAL ISCHEMIA/REPERFUSION INJURY IN RATS

作　　者：蒋鹏[1] 赵明[1] 刁玉晶[1] 谷腾飞[1] 马鹏 

机构地区：[1]江苏大学附属医院麻醉科,镇江212001

出　　处：《现代预防医学》2011年第7期1340-1341,1348,共3页Modern Preventive Medicine

基　　金：江苏省镇江市社会发展科技支撑项目(SH2010014)

摘　　要：[目的]研究参麦注射液对心肌缺血再灌注损伤大鼠细胞间粘附分子-1(ICAM-1)表达的影响。[方法]取30只SD大鼠随机分为假手术组(sham组)、心肌缺血再灌注未干预组(MI/R组)、参麦注射液组(S组),分组后S组每天注射参麦注射液10ml/kg连续3d,sham组及MI/R组则注射等量的生理盐水。按照心肌缺血再灌注损伤模型开胸结扎冠状动脉左前降支,造成心肌缺血30min后放松再灌注90min,建立心肌缺血再灌注模型。免疫组化法检测心肌细胞ICAM-1的表达。[结果]与sham组相比,心肌细胞ICAM-1蛋白的表达明显升高。S组与IR组相比,心肌细胞ICAM-1蛋白的表达明显降低。[结论](1)心肌细胞ICAM-1蛋白的表达升高;(2)参麦注射液能明显抑制ICAM-1表达上调;(3)参麦注射液对缺血再灌注大鼠心肌有保护作用,这种作用与抑制这种作用与抑制ICAM-1表达上调有关。[Objective]To investigate the effect of Shenmai injection on myocardial ischemia/reperfusion injury in rats.[Methods]30 SD rats were randomly divided into three groups：sham group（operation group）,MI/R group（ischemia-reperfusion without any intervention）,S group（administered with Shenmai injection）,then 10 ml·kg-1 Shenmai injection were given in Shenmai group and the 0.9% NaCl 10 ml·kg-1 were given in sham group and MI/R group for three days.After thoracotomy and ligation,the rats were injured by ischemia and relaxed 30 minutes later,then with 90 minutes reperfusion,the myocardial ischemia-reperfusion models in rats were established during the experiment.The ICAM-1 protein expressions were evaluated by immunohistochemistry.[Results]Comparing with sham group,the expression levels of ICAM-1 protein in myocardium were increased significantly.Comparing with MI/R group,the expression levels of ICAM-1 protein in myocardium were decreased significantly.[Conclusion]These data suggest that：（1）The expressions of ICAM-1 are increased significantly;（2）Shenmai injection can greatly depress expression of ICAM-1;（3）Shenmai injection may protect myocardium ischemia/reperfusion injury,down-regulated expression of ICAM-1 may be the possible therapeutic mechanisms.

关 键 词：缺血/再灌注损伤 细胞间粘附分子-1 参麦注射液 

分 类 号：R743.31[医药卫生—神经病学与精神病学]