聚丙烯酰胺降解菌株Enterobacter cloacae的分离鉴定  

Isolation and identification of hydrolyzed polyacrylamide (HPAM) degrading bacteria strain Enterobacter cloacae

作　　者：张兴福[1] 向阳[1,2] 徐德会[3] 陈忠喜[3] 

机构地区：[1]成都理工大学能源学院,成都610059 [2]中国石油大庆油田公司开发部,黑龙江大庆163453 [3]大庆油田建设设计研究院水化室,黑龙江大庆163712

出　　处：《哈尔滨商业大学学报（自然科学版）》2010年第6期671-674,共4页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：国家自然科学青年基金(50908063)

摘　　要：应用厌氧Hungate技术,从大庆油田聚合物配注站的熟化罐水溶液中分离到一株聚丙烯酰胺降解菌株.对该菌株进行形态、生理生化、分子生物学鉴定结果表明,菌株为G-,短杆状,无芽孢,最佳pH值为8.0,最适生长温度为40℃,具有硫酸盐还原功能,产H2S,严格厌氧,通过16S rDNA和16S^23S rDNA间隔区序列鉴定,菌株与Enterobacter cloacae有极高的相似性.初步鉴定为Enterobact-er中的新种,暂时命名为Enterobacter cloacae.红外光谱分析结果表明,菌株以聚丙烯酰胺为惟一碳源,菌株作用前后表面结构发生变化,分子链上的酰胺基水解成羧基,降解侧链,部分官能团发生改变,溶液黏度下降效果显著;GC-MS初步分析聚合物发生断链,低分子质量化合物除含双键、环氧和羰基的聚丙烯酰胺碎片外,大多属于一般丙烯酰胺低聚体的衍生物.A hydrolyzed polyacrylamide（HPAM）-degrading bacteria strain is isolated from curing pot in HPAM distribution station of Daqing oil field using Hungate anaerobic technique.The isolates is characterized morphologically,physiologically,biochemically and molecularly.It is gram negative,short bacillus,non-spore-forming,with an optimum at pH 8.0,with an optimum growth temperature of 40 ℃,strictly anaerobic bacteria,which can reduce sulfate to H2S.Alignment of 16S ribosomal DNA and 16S^23S ribosomal DNA intergenic spacer sequences suggests that this isolate is closely related to the Enterobacter cloacae.The isolate is identified as a new genus belongs to Enterobacter,temporarily named Enterobacter cloacae I7.Analysis results of Infrared Spectroscopy（IR） shows that the bacteria can use HPAM as only carbon source,making HPAM polymer surface structure changed,and hydrolyzing amide to carboxyl group by the molecule chain amido hydrolysis,degrading the side-chain and changing some function group,resulting in obviously decreased viscosity.Using GC-MS,the determined low-molecular weight compounds of HPAM degrading products are polyacrylamide fragments with duplet bond,epoxy as well as carbonyl group,but most of them are acrylamide oligomer derivatives.

关 键 词：聚丙烯酰胺降解菌 生物降解 ENTEROBACTER CLOACAE I7 系统发育 

分 类 号：X172[环境科学与工程—环境科学]