三叉神经脊束核尾侧亚核星形胶质细胞对咬合创伤的反应  

Response of astrocytes to occlusal trauma in spinal trigeminal subnucleus caudalis

作　　者：贾静[1] 刘雪梅[2] 章捍东[2] 刘洪臣[2] 

机构地区：[1]武警总医院口腔中心,副主任医师北京100039 [2]解放军总医院口腔医学中心,主治医师北京100853

出　　处：《口腔颌面修复学杂志》2010年第5期257-259,共3页Chinese Journal of Prosthodontics

基　　金：北京市自然科学基金(项目编号:7102165)

摘　　要：目的:观察大鼠咬合创伤后三叉神经脊束核尾侧亚核(Sp5C)内星形胶质细胞的反应,探讨星形胶质细胞在咬合创伤中的作用。方法:用方丝将大鼠左侧上颌第一磨牙咬合抬高0.5mm,形成左侧磨牙早接触的创伤咬合。粘固后1,3,7,14,30d取三叉神经脊束核尾侧亚核,免疫荧光染色观察星形胶质细胞在三叉神经脊束核尾侧亚核的表达变化。结果:对照组见少量胶质原纤维酸性蛋白(GFAP)阳性星形胶质细胞呈散在分布,咬合创伤3d时,实验侧GFAP阳性面积增加,荧光强度增强;7d时达到高峰,14d组GFAP阳性反应产物开始减少。结论:咬合创伤激活了星形胶质细胞,提示星形胶质细胞在口颌面痛产生及维持中发挥作用。Objective： To investigate the role of astrocyte in spinal trigeminal subnucleus caudalis（Sp5C）during occlusal trauma.Methods： The occlusal surface of the left first maxillary molars were raised 0.5mm with the square steel to establish traumatic occlusion animal models.On 1,3,7,14,30d after teeth operation,Sp5C were removed.Immunofluorescence histochemical was used to qualitively examine the distribution of GFAP in the Sp5C during the occlusal trauma.Results： Astrocytes in the Sp5C of control group occasionally had GFAP-IR.The number of GFAP-containing astrocytes increased in Sp5C at 3d after occlusal trauma,the maximum density of GFAP immunoreactivity in experimental side was found at 7d and decreased at 14d.Conclusion： The increase in numbers of astrocytes implied the possible involvement of astrocytes in traumatic occlusal pain.

关 键 词：咬合创伤 星形胶质细胞 胶质原纤维酸性蛋白 三叉神经脊束核尾侧亚核 

分 类 号：R782.2[医药卫生—口腔医学]