Sequence and Molecular Evolution Analysis of Ubiquitin Proteins Encoded by Baculoviruses  

杆状病毒泛素蛋白的序列及分子进化分析(英文)

作　　者：郭忠建[1] 朱颖敏[1] 陈克平[1] 

机构地区：[1]江苏大学生命科学研究院,江苏镇江212013

出　　处：《Agricultural Science & Technology》2010年第9期53-57,共5页农业科学与技术（英文版）

基　　金：Supported by China Postdoctoral Scientific Foundation(201004713-87);Natural Science Foundation for Universities of Jiangsu Province(07KJB180013);Foundation for Talented Man in Jiangsu University(05JDG048)~~

摘　　要：[Objective] The aim of this study was to analyze the sequence characteristics and molecular evolution of ubiquitins encoded by baculoviruses.[Methods]Clustal W software was used for multiple sequence alignment analysis,and neighbor-joining method（NJ）and maximum parsimony method（MP）were used for the construction of phylogenetic tree.[Results]The baculoviral ubiquitins showed 73%-86% sequence identity to eukaryotic ubiquitin.Two heterogeneous regions of baculoviral ubiquitins were observed：one was the residues from 15-32,the other was located from residues 53 to 60.The else parts were conserved,where many functional amino acids were also observed.Phylogenetic analysis indicated that baculoviral ubiquitins could be divided into three sub-families,including sub-family GV,sub-family I and sub-family II.The molecular evolution of baculoviral ubiquitins might be under negative selection to maintain their functional and structural stability.[Conclusion]The analysis had provided reference for the researches on functional characterization of baculoviral ubiquitins.[目的]分析杆状病毒泛素蛋白的序列特征和分子进化。[方法]运用ClustalW软件进行序列多重联配分析,采用临近法(NJ)和最大简约法(MP)构建系统进化树。[结果]昆虫杆状病毒泛素与真核生物泛素的一致性为73%～86%,差异体现在氨基酸15～32和53～57的序列上,其他序列高度保守。在真核生物泛素蛋白序列中,许多功能性的氨基酸残基在昆虫杆状病毒泛素蛋白序列中也存在。系统进化分析将昆虫杆状病毒和真核生物泛素蛋白大致分为3个亚家族,即GV族、I族和II族。杆状病毒泛素基因采取负选择的进化方式,在长期的进化过程中,其氨基酸序列倾向于逐渐被固定下来,达功能和结构上的稳定。[结论]分析杆状病毒泛素的序列特征和分子进化,为进一步研究其生物学功能提供参考。

关 键 词：BACULOVIRUS UBIQUITIN SEQUENCE Molecular evolution 

分 类 号：S852.65[农业科学—基础兽医学]