HPLC法串联FLD-VWD检测器测定食品中糖精钠的方法研究  

Detection of Saccharin Sodium in Foods by High-performance Liquid Chromatography with Fluorescence and Ultraviolet Detector

作　　者：刘锐萍 李志芬 马艳荣 

机构地区：[1]秦皇岛市产品质量监督检验所,河北秦皇岛066000 [2]秦皇岛市药品检验所,河北秦皇岛066000

出　　处：《中国食物与营养》2011年第3期54-56,共3页Food and Nutrition in China

摘　　要：利用高效液相色谱法(HPLC)将荧光检测器与紫外检测器串联检测食品中的糖精钠含量。该方法与常规的高效液相色谱法比较,增加了荧光检测器。两个检测器相互验证,能够避免单纯用紫外检测器由于食品中成分复杂常出现定性假阳性或定量偏高的情况。试验表明,相同条件下,荧光检测器的检出限比紫外检测器的检出限低4倍。串联后荧光检测器的回收率为99.71%,紫外检测器的回收率为99.62%。Researching the method to detect the saccharin sodium content in foods by the series connection of HPLC fluorescence and ultraviolet detector.By comparison with the common method,adding one fluorescence detector,the two detectors can authenticate each other,avoiding the false-positive qualitative or quantitative high.Experimentation manifested that the detecting limit of fluorescence detector is four times lower than ultraviolet detector.In series connection,the recovery rate of fluorescence detector is 99.71%,the recovery rate of ultraviolet detector is 99.62%.

关 键 词：串联检测器 荧光检测器-紫外检测器 糖精钠 高效液相色谱法 

分 类 号：TS207.3[轻工技术与工程—食品科学]