低温烷烃降解菌Yarrowia sp.分离、鉴定及降解特性  

Isolation,Identification and Degradation Characterization of One Low Temperature Alkanes-Degrading Bacterium Yarrowia sp.

作　　者：马会强[1] 张兰英[2] 张洪林[1] 李爽[1] 

机构地区：[1]辽宁石油化工大学环境与生物工程学院,抚顺113001 [2]吉林大学环境与资源学院,长春130026

出　　处：《长春理工大学学报（自然科学版）》2010年第4期157-160,共4页Journal of Changchun University of Science and Technology(Natural Science Edition)

基　　金：国家863高技术研究发展计划项目(2007AA06Z343)

摘　　要：以柴油为唯一碳源,在10℃低温条件下,从长期受原油污染场地中,经低温富集、分离,筛选出1株能够高效降解地下水中柴油的A菌。通过18S rDNA碱基测序及系统发育分析,确定该菌属于耶氏酵母菌属(Yarrowiasp.)。降解实验表明:A菌具有广泛且有效的烷烃降解特性,对50mg/L正十五烷、正十六烷36h降解率为85%,可有效降解柴油中C12-C27正构烷烃、支链烷烃、环烷烃,且对姥鲛烷、植烷也具有一定降解能力。以上为该菌株在低温地区石油烃污染土壤及地下水生物修复中的应用奠定了基础。A bacterium was low temperature enriched,isolated and screened from one site contaminated by crude oil for a long time under 10℃.This strain was identified as Yarrowia sp.through 18S rDNA basic sequence detection and phylogenetic analysis.The results indicated that a bacterium have effective and broad-spectrum degradation capability of alkanes,and can degrade 85% of 50mg/L n-pentadecane and n-hexadecane for 36h,and also effectively degrade all of C12-C27 n-alkanes,branched alkanes,cycloalkanes,parts of pristane and phytane from diesel.This research lay solid foundation for a bacterium used in bioremediation of petroleum hydrocarbon contaminated soil and groundwater especially at low temperature regions.

关 键 词：低温 烷烃 降解 耶氏酵母菌 

分 类 号：X172[环境科学与工程—环境科学]