强雌性系西瓜ACC合成酶基因的克隆及其序列分析  

Cloning and Sequence Analysis of ACC Synthase Gene in Subgynoecious Watermelon

作　　者：胡宝刚[1] 刘莉[1] 焦定量[2] 

机构地区：[1]天津大学农业与生物工程学院,天津300072 [2]天津科润蔬菜研究所,天津300384

出　　处：《华北农学报》2011年第1期67-71,共5页Acta Agriculturae Boreali-Sinica

基　　金：天津市应用基础研究计划项目(07JCZDJC04200)

摘　　要：为了解ACC合成酶基因对西瓜花性型分化的作用机理,根据已报道的西瓜ACC合成酶(ACS)基因序列,设计特异性引物,以强雌性西瓜总RNA为模板,通过RT-PCR扩增得到4条特异性片段:Cit-ACS1为1 690 bp、Cit-ACS2为402 bp、Cit-ACS3为598 bp和Cit-ACS4为499 bp。序列同源性比对表明,该4个片段与已报道的西瓜ACC合成酶基因序列的同源性99.8%～100%。构建了西瓜ACC合成酶基因系统进化树,显示Cit-ACS1与甜瓜CMe-ACS2和黄瓜CS-ACS2亲缘关系最近;并对Cit-ACS1基因编码的蛋白进行了二级结构和三级结构分析。According to the reported ACC synthase（ACS）gene sequences of watermelon,specific primers to amplify ACS genes from subgynoecious watermelon by RT-PCR were designed in order to understand the mechanism of ACC synthase gene on flower sexual differentiation.PCR amplification produced four fragments with size 1 690 bp（Cit-ACS1）,402 bp（Cit-ACS2）,598 bp（Cit-ACS3）and 499 bp（Cit-ACS4）.Sequence homology analysis indicated that the identity between the four fragments and the reported watermelon ACS gene sequences were 99.8%-100%.A phylogenetic tree of ACS genes was constructed by software MEGA 4 and the similarity between Cit-ACS1 and CMe-ACS2,CS-ACS2 was the highest.Secondary and tertiary structure of the protein encoded by Cit-ACS1 gene were analyzed by bioinformatic databases.

关 键 词：西瓜 强雌性系 ACC合成酶 RT-PCR 

分 类 号：S651[农业科学—果树学] Q78[农业科学—园艺学]