大鼠肝纤维化中超声造影的实验研究  

Experimental study of contrast enhanced ultrasound in rats with liver fibrosis

作　　者：李宏波[1] 张炽敏[1] 庄华[1] 

机构地区：[1]东南大学医学院附属南京市第二医院超声诊断科,江苏南京210003

出　　处：《重庆医学》2011年第10期949-951,953,F0002,共5页Chongqing medicine

基　　金：南京市卫生局青年科技人才启动基金资助项目(QYK09153)

摘　　要：目的探讨超声造影在肝纤维化模型中的应用价值。方法 60只雄性大鼠,实验组52只建立大鼠肝纤维化模型,予50%四氯化碳(CCl4)植物油混悬液皮下注射,每周2次,共计12周,首次皮下注射剂量为0.5 mL/100 g体质量,以后为0.3 mL/100 g体质量,于3、6、9、12周末随机从造模大鼠中取10只,从对照组大鼠中取2只行超声造影检查,记录造影剂到达肝动脉时间(HAAT)、造影剂到达门静脉时间(PVAT)、造影剂到达肝静脉时间(HVAT),计算肝动-静脉渡越时间(HA-HVTT),进入CnTI程序,获取肝实质时间-强度曲线,记录达峰值时间、峰值强度、曲线上升斜率、下降斜率。以病理肝纤维化分期进行分组。结果随着肝纤维化程度的加重,S3期、S4期HVAT、HA-HVTT较对照组、S1期、S2期缩短。肝实质时间-强度曲线分析,S3期、S4期峰值强度及曲线下降斜率较对照组、S1期、S2期降低。结论应用超声造影可以观察大鼠肝动脉、门静脉、肝静脉显影过程,能反映肝纤维化各期的血流动力学变化趋势,为早期肝硬化的无创性诊断提供了新的可能。Objective To explore the value of contrast enhanced ultrasound in early diagnosis of liver fibrosis in rats. Methods Sixty SD rats were divided into two groups randomly,8 as control group,52 for liver fibrosis mode. 50% CC14 was percutaneously injected twice a week for 12 weeks at 0.3ml/100g with the first administration at 0.5ml/100g. At the 3 th,6 th,9 th,and 12 th week,10 rats from liver fibrosis group and 2 rats from control group were selected for CEUS,recording HAAT, PVAT, HVAT, and calculating HA-HVTT（HVAT-HAAT）. In CnTI program obtained the time-intensity curve of liver parenchyma and recorded the time to peak, peak intensity, ascending slope, descent slope. All rats were grouped according to pathological liver fibrosis stage. Results With the progress of liver fibrosis, HVAT, HA-HVTT was shorter in S3 and S4 stage than in control group, Sland S2 stage. The parenchyma time-intensity curve analysis showed that peak intensity and descent slope was lower in S3 andS4 stage than in control group,S1 and S2 stage. Conclusion CEUS can be used to observe the developing process of the hepatic artery, portal vein,hepatic vein and can demonstrate hemodynamic trend in different liver fibrosis stage. It also provides a new possibility for non- invasive diagnosis of liver cirrhosis.

关 键 词：肝硬化 超声造影 动物实验 

分 类 号：R575.2[医药卫生—消化系统]