TGF-β_1在C57BL/6N系小鼠腭突发育及腭裂形成中mRNA转录水平的改变和意义  

The mRNA transcription of TGF-β 1 during development of normal palate and cleft palate in C57BL/6N strain mouse

作　　者：李鑫[1] 金岩[1] 董绍忠[1] 朱萧玲[1] 

机构地区：[1]第四军医大学口腔医学院病理科,710032

出　　处：《现代口腔医学杂志》1999年第3期161-163,共3页Journal of Modern Stomatology

摘　　要：目的　通过观察 Ｔ Ｇ Ｆ－ β１ 在腭裂形成及腭突正常发育中的表达改变， 探讨其在腭突不同生长时期的作用。方法　利用建立的腭裂模型， 用原位杂交技术检测 Ｔ Ｇ Ｆ－ β１ 在ｍ Ｒ Ｎ Ａ 转录水平的变化。结果　 Ｔ Ｇ Ｆβ１在正常组 Ｒ Ｎ Ａ 表达比相同腭突生长期的腭裂组明显增高（ Ｐ ＜ ０ ．０１） 。正常组小鼠腭突发育至接触融合期， Ｔ Ｇ Ｆ－ β１ Ｒ Ｎ Ａ 表达与垂直期、上抬期之间也均有显著差异（ Ｐ＜ ０ ．０１） 。垂直期与上抬期之间无差异（ Ｐ＞ ０ ．０５） 。结论　 Ｔ Ｇ Ｆ－ β１ 是腭突间充质细胞增殖的正向调控分子。腭突发育后期， Ｔ Ｇ Ｆ－ β１ 对腭间充质细胞生长并趋于分化的调控作用明显增强。Objective To investigate the mRNA expression of TGF β in different developmental stage of mouse palate and in the formation of cleft palate. Methods The mRNA transcription of TGF-β 1 in the normal developmental palate and established cleft palate were observed by in situ hybridization method. Results The expression of TGF -β 1 was increased in the late stage of normal development of palate. Another interesting finding was that the expression of TGF-β 1 in the normal development of mouse palate was significantly higher than in the cleft palate during the same stage (P< 0.01 ). Conclusion The expression of TGF-β 1 is associated with the proliferation of mesenchymal cell and the growth of palate. TGF-β 1 is also a critical factor in inducing mesenchymal cell differentiation.

关 键 词：TGF-Β1 腭突发育 腭裂 mRNA 

分 类 号：R782.22[医药卫生—口腔医学]