黑果枸杞中鞣质含量测定方法的优化  

To Optimize the Content Determination Method for Tannin in Lycium ruthenicum Murr by UV

作　　者：孟庆艳[1,2] 马国财[2] 白红进[1,2] 

机构地区：[1]塔里木盆地生物资源保护利用兵团重点实验室,新疆阿拉尔843300 [2]塔里木大学生命科学学院,新疆阿拉尔843300

出　　处：《塔里木大学学报》2011年第1期9-14,共6页Journal of Tarim University

基　　金：塔里木盆地生物资源保护利用国家重点实验室培育基地开放项目(BRYB1005);新疆生产建设兵团科学技术局政策引导项目(2006YD29)

摘　　要：分析黑果枸杞果实中鞣质的含量。方法:用匀浆法提取黑果枸杞果实中鞣质;采用紫外-可见分光光度法于670 nm处测定鞣质的含量。结果:鞣酸在4.90-24.50μg.mL-1浓度范围内与吸光度有良好的线性关系,回归方程为Y=1.241 9X-0.042 8r,=0.999 9(n=5),黑果枸杞中鞣质的含量为3.03%,平均回收率为97.38%,RSD为1.11%(n=9)。结论:本方法简便、快速、准确,可用于黑果枸杞的鉴别和质量控制。Tannin was extracted from Lycium ruthenicum with Homogenization.The content of Tannin was determinated by UV at 670 nm wavelength.The paper analyzed content of Tannin in Lycium ruthenicum.The results showed that the calibration curve was linear with the concentration ranges of 4.90-24.50 μg ·mL-1（n=5）.The content of Tannin in Lycium ruthenicum is 3.03 %.The everage recovery was 97.38 %,with RSD of 1.11 %（n=9）.This way is handy、celerity、nicety.It can be used to discriminate and dominate the mass of Lycium ruthenicum Murr.

关 键 词：黑果枸杞 鞣质 紫外-可见分光光度法 含量测定 

分 类 号：TS207.7[轻工技术与工程—食品科学]