高致病性蓝耳病毒与低致病性蓝耳病毒RT-PCR检测方法  

Detection of High and Low Pathogenic Porcine Reproductive and Respiratory Syndrome Virus by RT-PCR

作　　者：顾海洋[1] 左文斌[1] 贺艳艳[1] 潘辉[1] 刘书东[1] 李莲瑞[2] 

机构地区：[1]塔里木大学生命科学学院,新疆阿拉尔843300 [2]新疆生产建设兵团塔里木畜牧科技重点实验室,新疆阿拉尔843300

出　　处：《塔里木大学学报》2011年第1期20-24,共5页Journal of Tarim University

摘　　要：将高致病性蓝耳病和低致病性蓝耳病病毒接种于Marc145细胞培养并观察细胞病变情况,将有细胞病变的病毒进行连续传代培养。根据高致病性蓝耳病与低致病性蓝耳病在NSP2基因上有87 bp连续缺失,从NCBI上下载7对高致病性蓝耳病保守序列设计一对引物。通过RT-PCR方法进行检测证明实验室所保存的这两株毒株分别为高致病性蓝耳病和低致病性蓝耳病,并且建立了高致病性蓝耳病与低致病性蓝耳病的检测方法。Marc145 cells was incubated with highly and lowly pathogenic porcine reproductive and respiratory syndrome virus to observe cytopathic effect.Then the cells with cytopathic effect were subcultured.According to the 87bp continuous gap between highly and lowly pathogenic porcine reproductive and respiratory syndrome virus in NSP2,a pair primers were designed by the conserved sequence of seven highly pathogenic porcine reproductive and respiratory syndrome virus sequences downloaded from NCBI.RT-PCR technology were used to demonstrate two viral strains were high and low pathogenic porcine reproductive and respiratory syndrome virus,respectively.Meanwhile the detection method of highly and lowly pathogenic porcine reproductive and respiratory syndrome virus were established.

关 键 词：蓝耳病 NSP2基因 RT-PCR 

分 类 号：S858.23[农业科学—临床兽医学]