马铃薯未授粉子房离体培养诱导双单体植株初探  

Induction of Dihaploid Plantlets of Potato Unpollinated Ovaries in Vitro

作　　者：张凤军[1] 张永成[1] 

机构地区：[1]青海省农林科学院/青海省马铃薯育种重点实验室,西宁810016

出　　处：《中国农学通报》2011年第1期144-148,共5页Chinese Agricultural Science Bulletin

基　　金：国家科技支撑"高产优质专用马铃薯育种技术研究及新品种选育"(2006BAD01A06-1-10);青海省重点科技攻关"马铃薯食品加工型新品种选育"(2006-N-129);国家马铃薯产业技术体系西宁综合试验站基金(zhsyz-27)

摘　　要：以MS培养基为基本培养基,添加不同种类和浓度的生长调节剂,离体培养8个马铃薯普通栽培种（2n=4x=48）品种的未授粉子房,获得了‘青薯168’和‘青薯7号’的双单体小植株。花蕾4℃下预处理24~72h的愈伤组织诱导率（51.24%~57.08%）比未预处理（9.35%）的明显提高。对‘青薯168’和‘青薯7号’而言,最佳培养基分别为MS＋2-4-D2.0mg/L＋ZT0.1mg/L＋蔗糖30g/L＋琼脂7g/L和1/2MS＋GA30.5mg/L＋2-4-D0.5mg/L＋KT2.0mg/L＋BAP2.0mg/L＋ZT0.2mg/L＋蔗糖20g/L＋琼脂7g/L。品种对愈伤组织分化形成小植株的影响比培养基的影响要大。Calluses and dihaploid plantlets were obtained from unpollinated ovaries of ‘ Qingshu168 ’ and ‘ Qingshu7 ’ by the in vitro culture. The buds were preprocessed at 4℃ for 24-72 h, and their callus induction rate was remarkably increased, comparing with that of non-pretreatment. The most suitable differentiation medium for ‘ Qingshu168 ’ was MS ＋ 2-4-D 2.0 mg/L ＋ ZT 0.1 mg/L ＋ sucrose 30 g/L ＋ agar 7 g/L, and for ‘ Qingshu7 ’ was 1/2 MS＋GA 3 0.5 mg/L＋2-4-D 0.5 mg/L＋KT 2.0 mg/L＋BAP 2.0 mg/L＋ZT 0.2 mg/L＋sucrose 20 g/L＋agar 7 g/L. It appeared that the plantlet differentiation mainly depended on cultivars.

关 键 词：马铃薯 未授粉子房培养 离体培养 双单体植株 

分 类 号：S532[农业科学—作物学]