高效液相色谱法快速测定黑花生种皮中花色苷含量  

Determination of Anthocyanin in Black Peanut Testae by HPLC

作　　者：黄荣峰[1] 杨素欣[1] 吴春霞[1] 韩立英[1] 王洪峰[1] 

机构地区：[1]山东师范大学生命科学学院/山东省逆境植物重点实验室,济南250014

出　　处：《中国农学通报》2011年第4期311-315,共5页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目"ZOU基因在调控胚胎发育中的功能研究"(30970278);山东省杰出青年基金项目"植物形态变异的系统生物学研究"(JQ200909)

摘　　要：黑花生花色苷具有很好的应用前景,为建立一个快速检测花生花色苷的方法,以黑花生和普通花生的种皮为材料,利用高效液相色谱测定了黑花生和普通花生种皮中花色苷的含量和主要成分。研究表明,黑花生种皮中花色苷的总含量为1.16mg/g鲜重。在高效液相测定过程中,其主要成分在保留时间10.283min和11.217min处有2个高吸收峰,它们的含量占总花色苷含量的98.3%。根据已有的研究结果,推断二者分别为矢车菊素-3-槐二糖、矢车菊素-3-接骨木二糖,其含量分别比普通花生高810倍和580倍。In order to establish a simple method to determine anthocyanin content of black peanut,which has great commercial potentials,we measured the content and major compositions of anthocyanin in both ordinary and black peanut testae by HPLC in this paper.Our results showed that there was 1.16mg/g total anthocyaninin fresh back peanut testae.Two major components,the content of which was 98.3%of total anthocyanin inback peanut testa,were detected as two peaks at 10.283 min and 11.217 min respectively during HPLC.They were 810 times and 580 times higher than ordinary peanuts’respectively,which were deduced as cyanidin-3-sophoroside and cyanidin-3-sambubioside according to previous study.

关 键 词：黑花生种皮 高效液相色谱法 花色苷 

分 类 号：Q946.83[生物学—植物学]