青蒿挥发油成分的GC-MS分析与化学计量学解析法  

Analysis of essential oil components in Artemisia annua by GC-MS combined with chemometric resolution method

作　　者：佘金明[1,2] 董红霞[2] 梁逸曾[1] 王玫[1] 谢峰[2] 

机构地区：[1]中南大学化学化工学院,中药现代化研究中心,湖南长沙410083 [2]常德职业技术学院药学系,湖南常德415000

出　　处：《中成药》2011年第1期99-103,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(20875104);湖南省教育厅科学研究项目(10C0055)

摘　　要：目的:分析青蒿挥发油的化学成分。方法:用水蒸气蒸馏法从青蒿中提取挥发油成分,采用气相色谱-质谱(GC-MS)联用技术,结合化学计量学解析法(CRM)对重叠色谱峰进行分辨解析,并利用程序升温保留指数辅助定性。结果:共分辨出73个色谱峰,鉴定了其中66个组分,占青蒿挥发油总含量的95.25%,其主要化学成分为:丁子香烯、丁子香烯环氧化物、雅槛蓝(树)油烯、α-甜没药萜醇等。结论:使用CRM解析色谱峰,比单独使用GC-MS法能更真实、全面地反映其挥发油化学成分。AIM：To analyze the essential oil components in Artemisia annua.METHODS：The volatile con-stituent was extracted from Artemisia annua by steam distillation.Gas chromatography-mass spectrometry（GC-MS） combined with chemometric resolution method（CRM）,a chemometric resolution method resolving overlapping peaks in chromatogram was employed to qualitative and quantitative analysis of the essential oil components,togeth-er with the temperature-programmed retention index for assistant identification.RESULTS：Total of seventy-three chromatographic peaks were resolved,and of these,sixty six components in the essential oils of Artemisia annua were identified,which accounted for 95.25% of the total relative contents.The main chemical compositions in the studied sample were identified as caryophyllene,caryophyllene epoxide,eremophilene,α-bisabolol etc.CONCLUSION：GC-MS with the help of CRM takes advantage over GC-MS without CRM reflecting really and comprehensively the essential oil components in Artemisia annua.

关 键 词：青蒿 挥发油 气相色谱-质谱 化学计量学解析法 

分 类 号：R284.1[医药卫生—中药学]