菊苣药材中菊苣多糖的含量测定研究  

Assay of Herba Cichorii polysaccharide content

作　　者：庄红艳[1] 张冰[1] 刘小青[1] 林志健[1] 吴丽丽[1] 

机构地区：[1]北京中医药大学中药学院,北京100029

出　　处：《中成药》2011年第1期114-117,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金资助项目(30472282);国家自然科学基金资助项目(81073063);北京市自然科学基金资助项目(7052036)

摘　　要：目的:建立菊苣多糖的含量测定方法。方法:采用紫外-可见分光光度法测定菊苣总糖、菊苣还原糖含量,计算得菊苣多糖的含量。结果:菊苣总糖含量测定中,果糖在0.006～0.016 mg/mL范围呈良好的线性关系,回归方程为Y=6.108X-0.026 6(r=0.999 1);菊苣还原糖含量测定中,果糖在0.06～0.11 mg/mL范围呈良好的线性关系,回归方程为Y=1.515 2X-0.258 9(r=0.999 2)。结论:本方法能简便有效的定量研究菊苣多糖,可用于完善菊苣药材的质量标准。AIM：To establish the mensuration of Herba Cichorii polysaccharide content.METHODS：Herba Cichorii polysaccharide total polysaccharide,and reducing sugar content in Herba Cichorii were determined through UV-VIS absorption spectrometry.RESULTS：Fructose in total polysaccharide was linear with its absorbance in the range of 0.006 mg/mL-0.016 mg/mL,and the regression equation was Y = 6.108X-0.026 6（r = 0.999 1）.Fructose in reducing sugar was linear with its absorbance in the range of 0.06 mg/mL-0.11 mg/mL,and the re-gression equation was Y =1.515 2X-0.258 9（r =0.999 2）.CONCLUSION：The method of measuring polysac-charide content in Herba Cichorii is convenient and effective,and can be used for the quality standard of Herba Ci-chorii.

关 键 词：菊苣 菊苣总糖 菊苣还原糖 菊苣多糖 含量测定 

分 类 号：R284.1[医药卫生—中药学]