HIV-1B’亚型及CRF07_BC重组毒株感染者中和反应特征分析  

Neutralization pattern of HIV-1 subtype B' and CRF07_BC virus chronically infected individuals

作　　者：胡新韬[1] 洪坤学[1] 赵春红[1] 郝晓培 郑扬[1] 任莉[1] 阮玉华[1] 邵一鸣 

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心传染病预防控制国家重点实验室,北京102206

出　　处：《中国热带医学》2011年第4期391-394,共4页China Tropical Medicine

基　　金：十一五重大传染病防治研究项目(2008ZX10001-010);传染病预防控制国家重点实验室课题(2008SKLID101)

摘　　要：目的探讨我国主要流行的HIV-1 B’和CRF07_BC重组毒株感染者中和反应特征。方法采用基于Env假病毒的以表达CD4及CCR5/CXCR4的TZM-bl细胞系为靶细胞的中和抗体测定方法,测定40例感染时间在3年左右的HIV-1 CRF07_BC重组毒株感染者及31例感染时间在10年以上的HIV-1 B’亚型毒株感染者血浆对11株包括B(4株)、CRF07_BC(3株)、CRF01_AE(3株)及中和敏感毒株(1株)Env假病毒的中和反应,并分析感染者血浆的中和宽度和中和强度。结果 B’亚型毒株感染者在中和宽度及亚型交叉中和强度方面均高于感染时间相对较短的CRF07_BC重组毒株感染者,B’亚型感染者对CRF01_AE亚型病毒的中和活性显著高于CRF07_BC重组型感染者;而CRF07_BC重组型感染者对同亚型病毒的中和强度则显著高于B’亚型感染者。结论 HIV-1感染者中和抗体宽度以及对异源病毒的中和活性与感染时间正相关,适度的病毒抗原刺激有助于广谱中和活性的产生。Aim To analyze the neutralizing antibody （Nab） responses to HIV-1 subtype B and CRFO7_BC virus in chronically infected individuals in China. Methods Total 71 anti-relroviral therapy-naive HIV-1 infected individuals were recruited in this study. Among them,40 cases were infected with CRF07_BC virus with infected time around 3 years and 31 cases were infected with subtype B virus wilh infected time over 10 years at the sampling time. The standardized Nab assay using Env pseudoviruses and TZM-bl cell-line expressing CD4 and CCR5/CXCR4 was performed against a panel of pseudoviruses composed of subtypes B（4）,CRF07_BC（3） and CRF01 AE strains and 1 neutalization-sensifive SF162.LS virus as control. Results The breadth and potency of Nab responses in subtype B virus chronically infected individuals against inter-subtype viruses were higher than that of CRF07 BC virus chronically infected individuals with shorter infected time. Subtype B virus chronical infection also exhibited higher neutralizing activities against CRF01_AE virus than that of CRF07_BC virus chronically infected individuals. Whereas CRF07_BC virus chronically infected cases showed higher neutralizing activities against subtype-matched viruses than subtype B virus chronically infected individuals. Conclusion Overall neutralization breadth and neutralizing activities against heterologous viruses evolve and extend over time during HIV-1 natural infection,and extended exposure to antigen may be beneficial for the development of broad-spectrum crossreactive neutralization antibody response.

关 键 词：人类免疫缺陷病毒 抗体 中和 假病毒 

分 类 号：R-331[医药卫生]