三氯乙烯对大鼠外周血淋巴细胞CD3+CD4+CD8+的影响  

Effect of trichloroethylene on the level of CD3+CD4+CD8+ lymphocyte in peripheral blood of rats

作　　者：黄新凤[1] 徐新云[1] 李学余[1] 刘月峰[1] 周丽[1] 

机构地区：[1]深圳市疾病预防控制中心,广东深圳518055

出　　处：《中国热带医学》2011年第4期461-463,共3页China Tropical Medicine

基　　金：深圳市科技计划项目资助(项目编号200802073)

摘　　要：目的探讨三氯乙烯(TCE)对大鼠外周血淋巴细胞CD3+CD4+CD8+的影响。方法采用豚鼠最大值法(GPMT),将大鼠随机分为3组,分别为阴性对照组、阳性对照组、TCE实验组,用皮内注射的方式分别注射橄榄油、2,4-二硝基氯苯(DNCB)、三氯乙烯(TCE),实验结束后收集大鼠外周血液,用流式细胞仪检测淋巴细胞CD3+CD4+CD8+比例。结果 TCE实验组和阳性对照组大鼠外周血淋巴细胞CD3+比例高于阴性对照组;阳性对照组CD4+、CD4+/CD8+高于阴性对照组,CD8+低于对照组,存在显著性差异(P<0.05);TCE实验组CD4+、CD8+、CD4+/CD8+与阴性对照组比较无统计学差异。结论三氯乙烯经皮染毒对大鼠外周血淋巴细胞亚型比例有一定影响,可能与三氯乙烯的致敏作用存在一定关系。Aim To investigate the effect of trichloroethylene （TCE） on level of CD3＋CD4＋CD8＋ lymphocyte in peripheral blood of rats. Methods Guinea pig maximization test （GPMT） was applied in this study, rats were divided into negative control group,positive control group and TCE treatment group. Animals in 3 groups were administrated with olive oil, 2,4-dinitrochlorobenzene （DNCB）, and TCE, respectively ,by intradermal injection. Peripheral blood was collected from the animals for determination of levels of lymphocyte CD3＋CD4＋CD8＋ with flow cytometer. Results The ratio of lymphocyte CD3＋ increased in TCE group and positive control group compared to negative control group. The ratio of CD4＋ and CD4＋/CD8＋ elevated in positive control group,but CD8＋ decreased in positive control group compared with negative control group. Additionally,the ratio of CD4＋ and CD4＋/CD8 ＋ did not show any variation in TCE group compared with negative control group. Conclusions Triehloroethylene treatment can affect the ratios of lymphocytes in peripheral blood of rats,it might be associated with trichloroethylene -induced hypersensitivity.

关 键 词：三氯乙烯 大鼠 外周血 淋巴细胞 CD3+CD4+CD8+ 

分 类 号：R-33[医药卫生]