电子垃圾拆解区新生儿脐带血淋巴细胞DNA损伤及其相关因素分析  

Analysis of DNA Damage of Umbilical Cord Blood Lymphocytes and Related Factors in Neonates from an E-waste Recycling Area

作　　者：王远萍[1] 霍霞[1] 肖琼娜[1] 李斌[1] 邱少汕[1] 刘俊晓[1] 徐锡金[1] 

机构地区：[1]汕头大学医学院分析细胞学实验室,广东汕头515041

出　　处：《汕头大学医学院学报》2011年第1期20-22,共3页Journal of Shantou University Medical College

基　　金：国家自然科学基金资助课题(30972513);高等学校博士学科点专项科研基金资助项目(200805600004)

摘　　要：目的:评估电子垃圾拆解区新生儿脐带血淋巴细胞DNA损伤状况,并分析其相关影响因素。方法:选择足月健康新生儿,15例来自电子垃圾拆解区—贵屿镇(暴露组),10例来自汕头市区(对照组)。通过单细胞凝胶电泳实验评估脐带血淋巴细胞DNA损伤,并根据调查问卷,分析相关影响因素。结果:暴露组脐带血淋巴细胞DNA较对照组损伤严重,尾长(21.9 vs 5.7),慧尾DNA百分含量(4.0 vs 1.0)及尾矩(1.7 vs 0.2)均高于对照组(P<0.001)。产妇生活在贵屿、产妇文化程度低、丈夫吸烟并从事电子垃圾拆解相关工作等6个因素与脐带血淋巴细胞DNA损伤呈正相关;产妇怀孕期间吃鱼、肉与脐带血淋巴细胞DNA损伤呈负相关。结论:贵屿镇新生儿DNA有不同程度的损伤,可能与当地电子垃圾拆解业所造成的环境污染有关。Objective： To analyze DNA damage of umbilical cord blood（UCB）lymphocytes and related factors in neonates from an e-waste recycling area.Methods： Full-term neonates were selected,15 from e-waste recycling area-Guiyu town（exposure group）and 10 from Shantou city（control group）.The single cell gel electrophoresis was done to evaluate DNA damage of UCB lymphocytes and questionnaires were used to analyze related factors.Results： Compared with the control group,there was increased DNA damage of UCB lymphocytes in the exposure group.The medians of tail length（21.9 vs 5.7）,the percent of tail DNA（4.0 vs 1.0）and tail moment（1.7 vs 0.2）in the exposure group were significant higher than those of control group（P0.001）.There was increased DNA damage of UCB lymphocytes in pregnant women who living in Guiyu or have lower educational level,whose husbands smoking or involved in e-waste.Fish and meat consumption of pregnant women were negatively correlated with DNA damage.Conclusion： DNA in neonates from Guiyu town is damaged at different extent,which may due to environmental pollution caused by e-waste recycling.

关 键 词：DNA 新生儿 电子垃圾污染 

分 类 号：R394.6[医药卫生—医学遗传学]