piggyBac转座系统在哺乳动物及其细胞中的研究进展  

Current advance of piggyBac in mammals and mammalian cells

作　　者：谢飞[1,2] 蒋世忠[1,2] 马晴雯[1,2] 

机构地区：[1]上海市儿童医院医学遗传研究所,上海200040 [2]上海交通大学医学院,上海200025

出　　处：《生命科学》2011年第3期255-260,共6页Chinese Bulletin of Life Sciences

基　　金：国家重点基础研究发展计划("973"项目)(2010CB529902)

摘　　要：piggyBac(PB)转座系统来源于昆虫鳞翅目,属于真核生物的第二类转座系统,主要采取"剪切粘帖"机制发生转座。PB系统转座效率高,宿主范围广,广泛应用于昆虫等低等生物的基因转移及突变筛选。近年来,研究发现PB系统在哺乳动物及其细胞中也具有高效的转座活性,已在动物基因组功能研究、基因转移及诱导多能干细胞等领域得到了广泛应用。本文就PB系统近年来在哺乳动物及其细胞中的研究进展、应用前景及存在问题进行了综述。piggyBac（PB） transposon,a TTAA-specific transposon firstly isolated from the genome of cabbage looper moth,is a member of class II transposon family,which jumps via a ＂cut and paste＂ mechanism.PB transposon has been widely used for insertion mutagenesis and carrying gene in lower organism.Recent studies demonstrate that PB transposon system possesses high activity of delivering large pieces of DNA in mammals and cultured mammalian cells and can remobilize efficiently without any track.These new findings accelerate the investigation about PB transposon system in transgenesis,gene therapy,functional genomics and generation of induced pluripotent stem cell（iPSC）.In this review,we summarized the recent progresses of PB in mammalian and cultured mammalian cells,and discussed the obstacles in application of piggyBac system.

关 键 词：PIGGYBAC 转座 哺乳动物 转基因 

分 类 号：Q78[生物学—分子生物学]