Ethanol extract from radix of Actinidia chinensis and Evodiamine regulated CCR7 expression in SW480 cells  

作　　者：Yan-qin Zhou Zhi-qun Zhou Hui-yin Li Ting-ting Guo Guo Chen Ying He Li-qun Kang Yu-xiang Chen 

机构地区：[1]School of Pharmacy, Central South University, Changsha, Hunan 410013, P.R. China

出　　处：《中国现代医学杂志》2011年第3期336-347,共12页China Journal of Modern Medicine

基　　金：supported by Hunan Natural Science Foundation(No:2009FJ3029);Changsha High-tech Project(No:K0902033-31).

摘　　要：Objective To identify anti-tumor effects of the ethanol extract from radix of Actinidia chinensis(EERAC) and Evodiamine(Evo) on colon carcinoma SW480 cells,and investigate their regulation of CCR7 expression and the underlying mechanism in colon carcinoma.Methods Radix of Actinidia chinensis was extracted by ethanol.MTT assay and growth curve method were used to detect the inhibition of SW480 cells after being treated with EERAC and Evo.RT-PCT and Western-blot were used to detect the CCR7 expression regulated by EERAC and Evo at the level of protein and mRNA respectively in SW480 cells.Results MTT results showed that EERAC and Evo significantly inhibited the growth of SW480 cells with obvious time-and dose-dependent effect.The 50% inhibitory concentration(IC50) of EERAC was 9.14 mg/mL,while the IC50 of Evo was 2.11 μg/mL.RT-PCR results showed that the expression of CCR7 mRNA in EERAC and Evo group was 29.99% and 18.03% respectively,which was obviously lower than that(56.27%) in control group.Western-blot proved that the expression of CCR7 protein in EERAC and Evo group was 29.03% and 15.28% respectively,which was also significantly reduced when compared to that of 42.48% in control group.Conclusion Ethanol extract from radix of Actinidiae chinensis and Evodiamine could effectively inhibit proliferation and growth of SW480 cells,significantly down-regulate the functional expression of CCR7,and thereby suppress the tumor metastasis.Objective To identify anti-tumor effects of the ethanol extract from radix of Actinidia chinensis （EERAC） and Evodiamine （Evo） on colon carcinoma SW480 cells, and investigate their regulation of CCR7 expression and the underlying mechanism in colon carcinoma. Methods Radix of Actinidia chinensis was extracted by ethanol. MTT assay and growth curve method were used to detect the inhibition of SW480 cells after being treated with EERAC and Evo. RT-PCT and Western-blot were used to detect the CCR7 expression regulated by EERAC and Evo at the level of pro- tein and mRNA respectively in SW480 cells. Results MTT results showed that EERAC and Evo significantly inhibited the growth of SW480 cells with obvious time- and dose-dependent effect. The 50% inhibitory concentration （ICs0） of EERAC was 9.14 mg/mL, while the IC^0 of Evo was 2.11 i^g/mL. RT-PCR results showed that the expression of CCR7 mRNA in EERAC and Evo group was 29.99% and 18.03% respectively, which was obviously lower than that （56.27%） in control group. Western-blot proved that the expression of CCR7 protein in EERAC and Evo group was 29.03% and 15.28% respectively, which was also significantly reduced when compared to that of 42.48% in control group. Conclusion Ethanol extract from radix of Actinidiae chinensis and Evodiamine could effectively inhibit proliferation and growth of SW480 cells, significantly down-regulate the functional expression of CCR7, and thereby suppress the tumor metastasis.

关 键 词：EERAC EVODIAMINE tumor metastasis CCR7 

分 类 号：R932[医药卫生—生药学]