卵巢上皮性癌肿瘤浸润淋巴细胞的增殖、表型及细胞毒活性研究  

STUDY OF CULTURE,PHENOTYPE,AND CYTOTOXICITY OF TUMOR INFILTRATING LYMPHOCYTES IN OVARIAN EPITHELIAL CARCINOMA

作　　者：吴小华 范金兰[1,2] 宋俊芬 王素君[1,2] 刘辉 

机构地区：[1]河北医科大学第四医院妇产科 [2]中国人民解放军第二军医大学

出　　处：《河北医科大学学报》1999年第5期282-285,共4页Journal of Hebei Medical University

基　　金：河北省科委资助项目

摘　　要：目的探讨卵巢上皮性癌（简称卵巢癌）肿瘤浸润淋巴细胞（ｔｕｍｏｒｉｎｆｉｌｔｒａｔｉｎｇｌｙｍｐｈｏｃｙｔｅｓ，ＴＩＬ）的生物学特性。方法用机械法和酶法消化实体瘤后，采用分步低速离心后行连续密度梯度法分离卵巢癌ＴＩＬ，在有ＩＬ２、ＰＨＡ或ＯＫＴ３条件下培养，表型分析采用间接免疫荧光染色法，细胞毒性检测采用ＭＴＴ法。结果１５份卵巢癌ＴＩＬ中，得率范围１０６～１０７／ｇ，１３份ＴＩＬ活化（另２份污染），最大扩增倍数５２３倍，其中７份ＴＩＬ经培养达治疗量（１０９个细胞）；初分离的卵巢癌ＴＩＬ处于功能抑制状态，抑制期５～１５天，随着活化后体外培养，ＣＤ３、ＣＤ８、ＣＤ２５（ＩＬ２Ｒ）和ＨＬＡＤＲ表达显著增多；初分离的卵巢癌ＴＩＬ对Ｋ５６２和自体瘤细胞杀伤力均低下，随着体外培养，细胞毒性增加，尤以对自体瘤的特异性杀伤力增加最显著。结论卵巢癌ＴＩＬ体外易分离及活化，特异性杀伤力较强。ObjectiveTo explore the biological characteristics of tumor infiltrating lymphocytes (TIL) in ovarian epithelial carcinoma (OVEC).MethodsOvarian cancer tissues were digested enzymatically or mechanically,then cultured in the presence of IL 2,PHA and OKT 3 mAb.The changes in phenotypes and cytotoxicities of TIL in OVEC were examined by indirect immunofluorescence method and MTT method separately.ResultsAll TIL in OVEC were activated and proliferated after 5～15 days' supression,during that peroid the abilities both in proliferatioin and cytotoxicity were poor.The most expanding flod was 532.OKT3mAb+IL 2 could induce higher expanding folds of TIL.The cytotoxities against K562,Raji and autologous tumor cells increased while TIL were cultured in vitro,and the increase of cytotoxicity against autologous tumor cells was most significant in TIL.Phenotypic analyses showed the expression of CD3,CD 8,CD 25 and HLA DR were increased in the process of cultivation and were correlated to the cytotoxicity.ConclusionTIL in OVEC has strong autologous tumor cytotoxicity and is potentially useful in clinical adoptive immunotherapy.

关 键 词：卵巢肿瘤 淋巴细胞 肿瘤浸润 细胞毒性 

分 类 号：R737.31[医药卫生—肿瘤]