声门上型喉癌中端粒酶活性的检测及其意义  

The detection and clinical significance of telomerase activity in supraglottic carcinomas

作　　者：魏宏权[1] 郭星[1] 付伟能[1] 孙开来[2] 

机构地区：[1]中国医科大学附属第一医院耳鼻咽喉科,沈阳110001 [2]中国医科大学医学遗传学教研室

出　　处：《临床耳鼻咽喉科杂志》1999年第9期387-389,共3页Journal of Clinical Otorhinolaryngology

基　　金：国家自然科学基金

摘　　要：目的：探讨端粒酶活性在声门上型喉癌中的表达及其意义。方法：采用端粒重复序列扩增法检测了 Ｈ Ｅ Ｐ２ 喉癌细胞系，２６ 例声门上型喉癌组织和１５ 例癌旁组织。结果： Ｈ Ｅ Ｐ２ 喉癌细胞系呈端粒酶阳性；癌组织的端粒酶阳性率为８４．６％ ，明显高于癌旁组织的阳性率４０％ （ Ｐ ＜ ０．０１）。喉癌组织端粒酶活性的表达与临床分期和有无颈淋巴结转移未表现出相关性（ Ｐ ＞ ０．０５）。结论：端粒酶活化是喉癌发生的重要遗传学改变。Objective:To study the expression and significance of telomerase activity in laryngeal carcinoma.Method:We detected telomerase activity of HEP 2 cell line,26 cases of supraglottic carcinoma tissues and 15 cases of peri carcinoma tissues with PCR based assay designated TRAP(for telomeric repeat amplification protocol).Result:The telomerase positive rate of supraglottic carcinoma 84.6 %(22/26) was obvious higher than that of peri carcinoma 40%(6/15),P< 0.01 .No significant differences in positive rates were found among different groups divided according to clinical stage or metastasis of cervical lymph node,P> 0.05 .Conclusion:Telomerase activation is related to immortalization process,though not much helpful in diagnosis of supraglottic carcinoma.

关 键 词：喉肿瘤 端粒酶 端粒 重复序列扩增法 

分 类 号：R739.65[医药卫生—肿瘤]