大鼠脑多梗死性痴呆模型的建立  

Establishing the Rat Model of Multi-infarct Dementia

作　　者：武冬慧[1,2] 胡金凤 李志鹏 吉海杰 陈乃宏 

机构地区：[1]中国医学科学院药物研究所药理室,北京市100050 [2]天津中医药大学,天津市300193

出　　处：《中国康复理论与实践》2011年第3期232-234,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金重点项目(No30973887;NoU832008);创新药物研究开发技术平台建设(No2009ZX09303;No2009ZX09301)

摘　　要：目的建立大鼠脑多梗死性痴呆（MID）模型。方法从大鼠颈内动脉逆行注入4mg/ml、8mg/ml葡聚糖凝胶微粒。术后24h对大鼠行行为学评分,术后26~30d行Morris水迷宫实验以及尼氏染色。结果 8mg/ml组动物行为学评分显著高于4mg/ml组;后续试验中模型组选取8mg/ml组。水迷宫实验模型组大鼠逃避潜伏期与空白组以及假手术组相比明显延长（P〈0.01）,穿越目标区域的次数明显减少（P〈0.01）。尼氏染色可观察到模型组固缩凋亡的尼氏体。结论葡聚糖凝胶能制备大鼠多梗死性痴呆。Objective To establish the rat model of multi-infarct dementia. MethodsSephadex （4 mg/ml, 8 mg/ml） was injected into the internal carotid artery through the common carotid artery. Neurological deficits were measured 24 h after the operation, and Morris water maze test as well as Nissl stain were observed 26~30 d after the operation. ResultsThere was significant difference between the model groups injected sephadex of 8 ml/ml and 4 mg/ml in the neurologic deficits. In the following experiment, the rats injected sephadex of 8 mg/ml were only used as model. For the water maze test, the escape latency was longer （P0.01） and the frequency across target area reduced （P0.01） in the model, while the apoptotic Nissl body could be observed. ConclusionA model of multi-infarct dementia could be established with the sephadex in rats.

关 键 词：多梗死性痴呆 葡聚糖凝胶 模型 大鼠 

分 类 号：R743.32[医药卫生—神经病学与精神病学]