肿瘤转移抑制基因nm23/NDPK、p53和癌基因p21及增殖细胞核抗原的表达与胃癌浸润、转移的关系  

Expression of nm 23/NDPK,p 53, p 21 and proliferating cell nuclear antigen in relation to the infiltration and metastasis in gastric cancer

作　　者：杜少陵[1] 彭敦发[1] 林鸿民[1] 

机构地区：[1]皖南医学院生物学教研室,241001

出　　处：《新医学》1999年第9期523-524,共2页Journal of New Medicine

基　　金：安徽省教委资助

摘　　要：目的：研究肿瘤抑制基因um23／NDPK、p53和癌基因p21及胃癌增殖细胞核抗原（PCNA）表达与胃癌浸润、转移的关系。方法：用链霉卵白素-生物素（SAB）免疫组织化学方法对55例经手术切除后的胃癌标本进行um23／NDPK、p53、p21和PCNA的检测。结果：55份胃癌标本中nm23／NDPK、p53、p21、PCNA阳性率分别为71％、29％、38％、84％，其中um23／NDPK、p21及PCNA表达与胃癌浸润深度有关，p21、PCNA阳性和nm23／NDPK表达降低者，癌细胞多已侵入浆膜及装膜外组织；um23／NDPK、p53、PCNA的表达与胃癌的淋巴结转移有关，um23／NDPK表达降低及p53、PCNA阳性者淋巴结转移明显增高（P＜0．05）。结论：胃癌组织中存在多种癌基因及抑癌基因表达异常，um23／NDPK的表过降低，p53、p21及PCNA阳性与胃癌浸润范围及淋巴结转移有关系。Objective: To study the expression of nm 23/NDPK,p 53, p 21 and proliferating cell nuclear antigen(PCNA) correlation with infiltration and metastasis in gastric cancer.Methods: The state of nm 23/NDPK, p 53, p21 and PCNA expression in fifty-five surgical samples of gastric carcinoma was investigated by means of streptavidin-biotin (SAB) immunohistochemical method. Results: The positive rates of nm 23/NDPK, p 53, p 21 and PCNA in 55cases of gastric cancers were 71%、29%、38% and 84 % respectively.The expression of nm 23/NDPK, p 21 and PCNAcorrelated to infiltration of gastric cancer. The cases with reduced expression of nm 23/NDPK and positiveness of p 21and PCNA had more trend to infiltrate serosa;and the cases with reduced expression of nm 23/NDPK and positivenessof p 53、PCNA had more trend to lymph nodes metastasis. P<0. 05. Conclusions:There are multiple abnormal expre-ssion of oncogenes and tumor suppressor genes in gastric cancers; The reduced expression of nm 23/NDPK, and posi-tiveness of p 53、p 21、PCNA have some relationships to infiltration and lymph nodes metastasis of gastric cancers.

关 键 词：抑癌基因 增殖细胞核抗原 胃癌 免疫组织化学 

分 类 号：R735.202[医药卫生—肿瘤]