NINJ2基因多态性及相关血清因子与卒中的相关性研究  

Relationship between stroke and both NINJ2 gene polymorphism and its related serum cytokines

作　　者：王欣[1] 张颖冬[1] 张宙[1] 

机构地区：[1]南京医科大学附属脑科医院神经内科,南京210029

出　　处：《中华神经医学杂志》2011年第2期115-121,共7页Chinese Journal of Neuromedicine

摘　　要：目的 研究中国汉族人群中NINJ2基因多态性与卒中的相关性以及肿瘤坏死因子-α（TNF-α）、神经生长因子（NGF）、白细胞介素-6（IL-6）、P-选择素（P-Selectin）在恢复期患者和正常人群中含量的差别. 方法 选择大动脉粥样硬化性（LAA）脑梗死患者52例、小动脉闭塞性（SAO）脑梗死患者85例、脑出血（ICH）患者50例及正常对照者66例,采用聚合酶链式反应-限制性片段长度多态性（PCR-RFLP）技术检测NINJ2基因两个SNP位点（rs12425791、rs11833579）的基因型,比较各组间的基因型及等位基因分布频率是否存在差异.采用多类结果变量的logistic回归分析法计算各患者组基因型的OR值,并给出95%CI.采用ELISA法检测TNF-α、NGF、IL-6及P-Selectin4种血清因子的含量,并比较组间及组内不同基因型间血清因子含量的差别. 结果 （1）对于rs12425791位点,LAA组及SAO组AG型频率及AA＋AG型频率明显高于对照组,比较差异有统计学意义（P＜0.05）,但ICH组与对照组比较差异无统计学意义（P＞0.05）;SAO组A等位基因频率明显高于对照组,比较差异有统计学意义（P＜0.05）,但LAA组及ICH组与对照组比较差异无统计学意义（P＞0.05）.对于rs11833579位点,各患者组的基因型及等位基因分布频率与对照组相比差异无统计学意义（P＞0.05）.（2）多类结果变量的logistic回归分析显示,在校正了其他危险因素的影响后,对于rsl2425791位点,LAA组AG型和SAO组AG型、AA＋AG型仍与卒中发病呈相关关系（其OR值分别为4.298、3.923及2.937,相应的95%C1分别为1.430～12.922、1.417～10.860及1.119～7.710）;而对于rs11833579位点,各患者组基因型与卒中发病无相关关系.（3）各患者组血清IL-6、TNF-α、NGF及P-Selectin含量与对照组相比差异无统计学意义（P＞0.05）.对于rs12425791位点,LAA组不同基因型间TNF-α含量差异有统计学意义（P＜0.05）,ICH组不同基因型间P-SObjective To investigate the relationship between NINJ2 gene polymorphism and stroke, and the differences of serum levels of tumor necrosis factor-αt （TNF-α）, NGF, interleukin-6 （IL-6）and P-Selectin in healthy controls and patients under recovery stage. Methods Fifty-two patients with large-artery atherosclerosis （LAA） infarction, 85 patients with small-artery occlusion lacunar （SAO）infarction, 50 patients with intracerebral hemorrhage （ICH） and 66 healthy controls were included in this study. Genotypes of the 2 single nucleotide polymorphism （SNP） sites （rs12425791 and rs11833579） in NINJ2 gene were examined by polymerase chain reaction-restriction fragment length polymorphism （PCR-RLFP） method. The differences of genotypes and alleles frequencies of the 2 SNP sites between each 2 different groups were analyzed and compared. Multinomial logistic regression was used to calculate the odds ratio （OR） of genotypes in each patient group, and 95% confidential interval （95% CI）was given. The serum levels ofTNF-α, NGF, IL-6 and P-Selectin were tested by ELISA method, and compared between groups and within group classified by genotypes. Results In regard to rs12425791 site, the frequencies of AG and AA＋AG genotypes in LAA and SAO groups were significantly higher than those in control group （P〈0.05）, while this difference was not found between the ICH group and control group （P〉0.05）; the frequency of A allele in the SAO group was significantly higher than that in the control group （P〈0.05）, while this difference was not found between the control group and both the LAA and ICH groups （P〉0.05）. In regard to rs11833579 site, no significant differences in the genotypes and alleles were noted between all the patient groups and control group （P〉0.05）. After adjusting the influence of other risk factors, the multinomial logistic regression analysis showed that the onset of stroke was still significantly associated with the AG genotype at rs12425791 site

关 键 词：NINJ2基因 多态性 单核苷酸 卒中 肿瘤坏死因子-Α 神经生长因子 白细胞介素-6 P-选择素 

分 类 号：R743.3[医药卫生—神经病学与精神病学]