养血清脑颗粒和丙戊酸联用对戊四氮点燃癫痫大鼠的影响  

Effect of Yangxue Qingnao granule combined with valproate on pentylenetetrazole-kindled epileptic rats

作　　者：谢媛媛[1] 连亚军[1] 

机构地区：[1]郑州大学第一附属医院神经内五科,郑州450052

出　　处：《中华神经医学杂志》2011年第2期142-146,共5页Chinese Journal of Neuromedicine

摘　　要：目的 探讨养血清脑颗粒（YXQNG）联用丙戊酸（VPA）对戊四氮（PTZ）慢性点燃模型大鼠癫痫发作、EEG、认知功能及颞叶、海马T型Ca2＋通道蛋白（Cav3.2）表达的影响. 方法 成年雄性SD大鼠40只按随机数字表法分为PTZ组、VPA组、VPA＋YXQNG组、NS组,每组10只.前3组大鼠腹腔注射PTZ溶液制作慢性点燃模型,VPA组大鼠在注射PTZ前1 h给予VPA灌胃;VPA＋YXQNG组除给予VPA外,注射PTZ前1.5 h给予YXQNG灌胃;NS组腹腔注射生理盐水,每天一次.8周后观察各组大鼠的行为学变化、Y型电迷宫检查大鼠的正确反应率、捕记EEG并应用免疫组化染色检测颞叶和海马Cav3.2的表达. 结果 给药8周后PTZ组大鼠全部达到完全点燃（连续3 d出现Ⅳ级发作或达到Ⅴ级发作）,VPA组和VPA＋YXQNG组大鼠仅出现0～Ⅱ级发作;Y型电迷宫检查结果显示VPA＋YXQNG组大鼠正确反应率高于PTZ组,差异有统计学意义（P＜0.05）;EEG结果显示PTZ组大鼠癫痫发作时EEG有明显异常放电,总功率高于用药前,差异有统计学意义（P＜0.05）.VPA组、VPA＋YXQNG组大鼠用药前后EEG总功率的差值均高于PTZ组,差异有统计学意义（P＜0.05）;免疫组化染色结果显示VPA组、VPA＋YXQNG组大鼠颞叶和海马Cav3.2表达低于PTZ组,VPA＋YXQNG组大鼠颞叶和海马Cav3.2表达低于VPA组,差异均有统计学意义（P＜0.05）. 结论 YXQNG和VPA联用能降低癫痫大鼠发作级别、改善认知功能、减少脑部异常放电并降低脑组织Cav3.2水平,有抗癫痫和脑保护作用.Objective To explore the effet of Yangxue Qingnao granule （YXQNG） on seizures and cognition function of pentylenetetrazole （PTZ）-kindled chronic epileptic rats models, expression of Cav3.2 in the hippocampus and the temporal lobe of these rats, and EEG features of the rats. Methods Forty healthy adult male SD rats were equally divided into 4 groups at random： PTZ group, VPA treatment group, VPA＋YXQNG treatment group, normal saline （NS）-control group （n=10）. PTZ solution was intraperitoneally injected for 8 weeks to induce the kindling model in the above 3 groups except the NS-control group. VPA by intragastric administration was given to the rats in the VPA treatment group 1 h before PTZ injection; YXQNG and VPA by intragastric administration were given to the rats in the VPA＋YXQNG treatment group 1.5 h before PTZ injection. Behavioral changes of the rats were observed 8 weeks after PTZ injection; accuracy rate of response of the rats were examined by electric maze test;EEG was performed; and the expression ofT-type Ca2＋ channel protein （Cav3.2） in the temporal lobe and hippocampus was detected by immunohistochemical staining. Results Rats in the PTZ group appeared grade Ⅳ or Ⅴ seizures for 3 consecutive d, and rats in the VPA treatment group, VPA＋YXQNG treatment group appeared grade 0-Ⅱ seizures. The accuracy rate of response of the rats in the VPA＋YXQNG treatment group was significantly higher than that in the PTZ group （P〈0.05）. EEG indicated that paradoxical discharge was noted in rats of PTZ group when seizures appeared, and the total power at the time was obviously higher than that before PTZ injection （P〈0.05）. The D-value of total power of EEG in rats of the VPA treatment group and VPA＋YXQNG treatment group before and after treatment was significantly higher than that in the PTZ group （P〈0.05）. And the level of Cav3.2 in the temporal and hippocampus in rats of the VPA treatment group and VPA＋YXQNG treatment group was significantly lower than that

关 键 词：癫痫 丙戊酸 养血清脑颗粒 

分 类 号：R742.1[医药卫生—神经病学与精神病学]