Lasp1 mRNA和蛋白在口腔鳞癌组织中表达上升及其临床意义  

Increased Expression of Lasp1 mRNA and Protein in Oral Squamous Cell Carcinoma Tissues with Their Clinical Significance.

作　　者：魏魁杰[1] 潘红芽[2] 韩伟[2] 杨筱[2] 叶冬霞[2] 钟来平[2] 张志愿[2] 

机构地区：[1]湖州师范学院医学院口腔系,浙江湖州313000 [2]上海交通大学医学院附属第九人民医院口腔颌面外科

出　　处：《口腔医学研究》2011年第1期37-39,43,共4页Journal of Oral Science Research

基　　金：国家自然科学基金资助项目(编号:30630065);浙江省自然科学基金资助项目(编号:Y2091171)

摘　　要：目的:探讨Lasp1基因与蛋白质水平在口腔鳞状细胞癌(OSCC)组织标本中的表达及其临床意义。方法:分别采用荧光实时定量RT-PCR和免疫组织化学方法,检测Lasp1在30例口腔鳞癌患者的癌组织及癌旁组织标本中mRNA和蛋白质表达水平,其表达水平与临床病理指标之间的关系采用SPSS10.0软件包进行统计分析。结果:口腔鳞癌组织标本中Lasp1 mRNA和蛋白质表达水平均显著高于癌旁组织(P<0.01)。Lasp1蛋白在临床晚期、淋巴结转移阳性病例中表达明显升高,与烟酒嗜好、肿瘤大小及肿瘤分化程度无关。结论:Lasp1在口腔鳞状细胞癌中表达上调,可能与肿瘤的发生、发展和淋巴结转移有关,可以作为一个潜在的生物标志物和治疗的靶标。Objective： To investigate the gene transcript level and protein expression of Lasp1 in oral squamous cell carcinoma tissues as well as their clinical significance.Methods： The mRNA level and protein expression of Lasp1 in 30 cases of oral squamous cell carcinoma tissues and paracancerous tissues were detected by fluorescent quantitive real-time RT-PCR and immunohistochemistry techniques respectively.The relationship between the Lasp1 expression level and the clinicopathological parameters was analyzed by SPSS10.0 software package.Results： The Lasp1 mRNA transcript level and protein expression in cancerous tissues increased significantly compared to the paracancerous tissues.Though no significant correlation was found between Lasp1 expression and smoking,drinking,T stage and pathological differential degree,OSCC patients with more advanced clinical stages and lymph node metastasis were prone to have higher expression of Lasp1.Conclusion： The results suggest that increased expression of Lasp1 contributes to cancerous progression and lymph node metastasis,and Lasp1 may be potentially used as a biomarker and a therapeutic target for OSCC.

关 键 词：口腔鳞状细胞癌 Lasp1 基因表达 实时定量RT-PCR 免疫组织化学 

分 类 号：R739.81[医药卫生—肿瘤]