猫视神经连续切片的计算机三维重建  

Computerized 3D reconstruction of cat optic nerve using serial sections

作　　者：高静琰[1] 蒋启达[1] 张美伦[1] 肖虹蕾[1] 佘振珏[1] 戴培东[2] 周国民[1] 

机构地区：[1]复旦大学上海医学院解剖与组织胚胎学系,上海200032 [2]复旦大学附属眼耳鼻喉医院

出　　处：《解剖学研究》2010年第6期435-437,F0004,共4页Anatomy Research

基　　金：国家973项目(2005CB724306);国家自然科学基金(30971536)

摘　　要：目的制备猫视神经的连续切片,选择合适的染色方法 ,寻找合适的配准方法 ,经图像采集及初步处理后利用计算机的三维重建技术,获得可视化的猫视神经三维结构。方法利用石蜡切片技术的制备猫视神经连续切片,采用特殊的神经三色染色法进行切片染色。利用数码显微镜及Motic Images Advanced 3.0和Motic Images Assembly 1.0软件对连续的猫视神经石蜡切片进行拍照,获得猫视神经连续切片的原始图像,将原始图像在GIMP软件中经过调整亮度对比度、初步图像配准、提取神经束膜图像信息后在Amira软件下,经过计算机在手工配准的基础上进一步自动配准,把二维的切片图像重建成三维的可视化图像。结果实验所得经三色染色的连续切片的原始图像上神经束膜能较好的与周围的组织区分;利用Amira三维重建软件,重建得到猫视神经三维结构图像,重建后的猫视神经结构三维图像可进行任意的切割、旋转、回复、拍照等操作。结论实验证明在不利用标志线的条件下,通过制备视神经连续切片,经过染色拍照,手工图像配准后利用计算机自动配准技术对视神经进行三维重建是完全可行的。Objective To prepare the serial sections of cat optic nerve,search for an exact method of staining and registration,and establish a visible virtual cat optic nerve model by the three-dimensional reconstruction program.Methods Based on the paraffin section method.The serial sections images were obtained by using Motic microscope and Motic software and saved as the format of JPEG.The three-dimensional model of cat optic nerve was reconstructed in computer by Amira 3.1.1 from the two-dimensional images.Results After staining,the perineurium can be separate from other tissue in most sections.The virtual cat optic never was succeeded reconstruction by using Amira 3.1.1.And the reconstructed cat optic nerve can be freely rotated and incised and restored.Conclusion Our study suggests that the method of without consult of registration in the main,and the method of 3D models reconstructed from serial histological sections of optic nerve is feasible.

关 键 词：视神经 三维重建 连续切片 三维图像 猫 

分 类 号：R77[医药卫生—眼科]