坐骨神经损伤早期背根神经节组织microRNA的表达变化  

Altered microRNA expression following sciatic nerve resection in the dorsal root ganglia

作　　者：于彬[1] 周松林[1] 钱天梅[1] 姚登兵[1] 丁斐[1] 顾晓松[1] 

机构地区：[1]南通大学神经再生重点实验室,江苏南通226001

出　　处：《解剖学研究》2010年第6期438-440,共3页Anatomy Research

基　　金：863重大项目(2006AA02A128);国家自然科学基金(30870811);江苏省自然科学创新学者攀登项目(BK2008010)

摘　　要：目的研究大鼠坐骨神经损伤以后短时间背根神经节组织中microRNA(miRNA)的表达变化。方法采用miRNA芯片检测神经损伤0 h(对照组)、3 h和9 h(实验组)背根神经节组织miRNA的表达情况,应用real time Taqman PCR对芯片结果进行验证。结果损伤3 h实验组与对照组比较发生上调和下调1.3倍以上的miRNA分别有4个和2个,损伤9h实验组与对照组比较发生上调和下调1.3倍以上的miRNA分别有7个和5个,real time Taqman PCR检测miR-188和miR-500的表达变化与芯片一致。结论大鼠坐骨神经损伤3h和9h后背根神经节组织中miRNA的表达谱发生改变。Objective To profile the expression of microRNAs（miRNAs） in the dorsal root ganglia（DRG） after resection of the sciatic nerve for short time in rats.Methods The expression of miRNAs in a sciatic nerve resection model at 0 hour,3 hours and 9 hours were examined by an Agilent rat miRNA array.The miRNA expression was confirmed by real time Taqman PCR.Results 4 and 7 miRNAs were significantly up-regulated in the DRG by 1.3-fold after 3 and 9 hours of resection,respectively;meanwhile,2 and 5 miRNAs significantly down-regulated.The expression of miR-188 and miR-500 analyzed by real time Taqman PCR was in agreement with the microarray data.Conclusion This study demonstrated a different involvement of miRNAs in the DRG after resection of the sciatic nerve in a rat model.

关 键 词：微RNA 神经损伤 背根神经节 

分 类 号：R745[医药卫生—神经病学与精神病学]