Olig2过表达对神经干细胞向少突胶质细胞分化的影响  

Effect of olig2 overexpression on differentiation of neural stem cells into oligodendrocytes

作　　者：吕合作[1,2,3] 陈治文[2] 胡建国[1,3] 

机构地区：[1]蚌埠医学院第一附属医院中心实验室,蚌埠233004 [2]蚌埠医学院生物化学与分子生物学教研室,蚌埠233030 [3]蚌埠医学院第一附属医院组织移植安徽省重点实验室,蚌埠233003

出　　处：《解剖学杂志》2010年第6期712-715,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金（30700439）;教育部科学技术研究重点项目（210103）;安徽省第5批（2010年度）优秀青年科技基金（10040606Y13）;安徽省教育厅自然科学基金（KJ2010B109）

摘　　要：目的：构建大鼠olig2真核表达载体,观察其过表达对大鼠神经干细胞（NSCs）向少突胶质细胞分化的影响.方法：采用 RT-PCR,以新生大鼠脊髓RNA为模板,扩增olig2基因,定向克隆到pEGFP-N3载体中.用电穿孔方法转染pEGFP-N3-olig2表达载体至NSCs中.用RT-PCR鉴定olig2的表达,用免疫荧光显色鉴定NSCs向少突胶质细胞的分化情况.结果：成功构建了pEGFP-N3-olig2真核表达载体;olig2在重组质粒转染的NSCs中能够高效表达;重组质粒转染的NSCs在体外诱导分化后,能够较空质粒转染的NSCs产生更多的受体相互作用蛋白（RIP）阳性细胞.结论：olig2过表达能够促进大鼠NSCs向少突胶质细胞方向分化.Objective： To construct rat olig2 eukaryotic expression plasmid and detect effect of its overexpression on differentiation of rat NSCs into oligodendrocytes. Methods： RT-PCR was used to amplify rat olig2 gene from RNA of neonatal rat spinal cord. The olig2 gene was inserted into the eukaryotic expression of vector pEGFP-N3 to construct the recombinant expression vector pEGFP-N3-olig2. The recombinant vector was transfected into NSCs by electroporation method. The expression of olig2 in NSCs was detected by RT-PCR. The differentiation of rat NSCs into oligodendrocytes was detected by iramunofluorescence staining. Results： The sequence of the cloned olig2 was confirmed to be correct by DNA sequencing. The NSCs transfected with pEGFP-N3-olig2 expressed olig2 efficiently. The pEGFP-N3-olig2 transfected NSCs differentiated into more receptor interaction protein （RIP） positive cells than the pEGFP-N3 transfected ones. Conclusion： Olig2 over-expression may significantly promote the differentiation of rat NSCs into oligodendrocytes.

关 键 词：olig2 神经干细胞 少突胶质细胞 基因表达 克隆 大鼠 

分 类 号：R774.6[医药卫生—眼科]