和厚朴酚联合硼替佐米对骨髓瘤KM3细胞增殖和凋亡的作用  

Effects of Honokiol and Bortezomib on proliferation and apoptosis in myeloma KM3 cells

作　　者：李珊[1] 李丽珍[1] 宋强[1] 赵川莉[1] 闫树昕[1] 王鲁群[1] 

机构地区：[1]山东大学齐鲁医院血液科,济南250012

出　　处：《山东大学学报（医学版）》2010年第12期32-36,共5页Journal of Shandong University:Health Sciences

摘　　要：目的探讨和厚朴酚(HNK)及硼替佐米对人多发性骨髓瘤KM3细胞增殖的影响及诱导凋亡作用。方法本实验分实验组和对照组(A组),实验组分为HNK单药组(B、C、D、E、F、G组的药物质量浓度分别为4、6、8、10、12、15μg/mL)、硼替佐米单药组(H、I、J组的药物质量浓度分别为5、10、20ng/mL)及二者联合用药组(K组为HNK4μg/mL+硼替佐米5ng/mL,L组为HNK4μg/mL+硼替佐米10ng/mL,M组为HNK4μg/mL+硼替佐米20ng/mL)。在不同的时间(24、48、72h)采用MTT比色法检测对照组和实验组细胞增殖活性;采用流式细胞术检测对照组和实验组细胞凋亡的变化。结果和厚朴酚4～15μg/mL对KM3细胞作用24、48、72h的细胞增殖抑制率分别由20.38%升至80.54%、27.45%升至89.99%、44.94%升至90.91%,不同组、不同作用时间相比差异均有统计学意义(P<0.05)。H组、I组、J组KM3细胞48h的细胞增殖抑制率分别是13.13%、36.22%、53.99%,各组间差异有统计学意义(P<0.05)。K组、L组、M组作用48h的细胞增殖抑制率分别是52.68%、69.99%、78.53%,K组与H组、L组与I组、M组与J组比较差异有统计学意义(P<0.05)。流式结果显示B组、C组、E组作用于KM3细胞24h和48h后细胞凋亡率分别是6.92%、16.15%、60.70%和18.84%、37.21%、86.07%,同一时间不同实验组之间差异有统计学意义(P<0.05)。H组、I组作用于KM3细胞24、48h的细胞凋亡率分别是9.27%、17.87%和11.92%、53.51%,同一时间不同实验组之间差异有统计学意义(P<0.05)。K组、L组作用于KM3细胞24、48h,细胞凋亡率分别是15.75%、22.18%和35.96%、74.70%,K组、L组细胞凋亡率明显高于H组、I组,差异有统计学意义(P<0.05)。结论和厚朴酚与硼替佐米均可诱导KM3细胞凋亡,抑制KM3细胞的增殖,两者联合作用能显著提高KM3细胞的凋亡率,增强抑制KM3细胞的生长。Objective To investigate the anti-proliferative and apoptosis effects induced by Honokiol（HNK）and Bortezomib on KM3 human multiple myeloma cell line in vitro.Methods Samples were divided into the control group and the experimental group,and the latter was further subdivided into the HNK treatment group（group B,C,D,E,F,G at respective concentrations of 4,6,8,10,12,15 μg /mL）,the Bortezomib treatment group（group H,I,J at respective concentration of 5,10,20 μg /mL）,and the combined treatment group（group K,L,M with HNK 4 μg/mL ＋ Bortezomib 5 ng/mL,HNK4 μg/mL ＋ Bortezomib 10 ng /mL and HNK4 μg/mL ＋ Bortezomib 20 ng/mL,respectively）.MTT colorimetric assay were performed to assess cell proliferation and cell apoptosis was determined by PI and Annexin V fluorescent staining on flow cytometry.Results Honokiol,at concentrations between 4-15 μg/mL,showed significant inhibiting effect on KM3 cell proliferation.At 24 h,48 h,and 72 h,the inhibition rate rose from 20.38%,27.45% and 44.94% to 80.54%,89.99% and 90.91%,respectively.There were significant differences among different groups,or at different time points.The inhibition rates of Honokiol on KM3 cells at 48 h in H,I,J,K,L,M were 3.13%,36.22%,53.99%,52.68%,69.99%,78.53%,respectively.There were considerable differences between K and H,L and I,or M and J（P〈0.05）.Apoptosis rate of KM3 cells in B,C,E at 24h and 48 h were 6.92%、16.15%、60.70% and 18.84%、37.21%、86.07%,respectively.There were significant differences in apoptosis among different groups at the same time point（P〈0.05）.Apoptosis rate of KM3 cells in H and I group were 9.27% and 17.87% at 24 h,11.92% and 53.51% at 48 h.Statistical differences were found in apoptosis between H and I group（P〈0.05）.Apoptosis rates of KM3 cells in K and L group were 15.75% and 22.18% at 24h,35.96% and 74.70% at 48 h.The apoptosis rates in K and L group were significantly higher than H and I group（P〈0.05）.Conclusion Honokiol and Bortezomib can inhibit the proliferation of KM3 cell

关 键 词：和厚朴酚 硼替佐米 KM3细胞 生长抑制 凋亡 

分 类 号：R733.3[医药卫生—肿瘤]