E-cadherin诱导的卵巢上皮细胞恶变模型的建立及其特征  

Construction and characteristics of an E-cadherin induced cancer model from ovarian epithelial cells

作　　者：张红[1] 刘联[2,3] 董玲玲[2,3] 杜超[3] 马春红[3] 韩丽辉[3] 黎莉[2] 

机构地区：[1]山东大学齐鲁医院老年病科,济南250012 [2]山东大学齐鲁医院肿瘤内科,济南250012 [3]山东大学医学院免疫学研究所,济南250012

出　　处：《山东大学学报（医学版）》2010年第12期62-66,共5页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(30772329);中国博士后科学基金面上资助(20090450153);山东省自然科学基金(ZR2009CM004);山东省博士后创新项目专项基金(200801009)

摘　　要：目的建立E-cadherin诱导的卵巢上皮细胞早期恶变模型,并对其生长增殖等生物学行为进行初步研究。方法采用含人E-cadherin基因CDH1全长cDNA的质粒转染中国仓鼠卵巢(Chinese hamster ovary,CHO)细胞,利用G418筛选稳定转染细胞株,通过Western Blot验证其E-cadherin的表达,然后通过CCK8细胞增殖实验及集落形成实验检测其生物学行为的变化。结果 CHO细胞转染CDH1基因后,高度表达E-cadherin蛋白,生长增殖水平显著提高,并具备了恶性细胞特有的非锚定依赖性生长的能力。结论构建了E-cadherin诱导的卵巢上皮细胞恶变模型,为卵巢肿瘤发生机制的研究提供了可靠的工具。Objective To construct an E-cadherin induced cancer model from ovarian epithelial cells and do primary research on its biological behaviors such as proliferation and growth.Methods Chinese hamster ovary（CHO）cells were transfected by plasmid including whole human E-cadherin cDNA and stably screened with G418.Expression of E-cadherin was identified by Western Blot,and cell proliferation test with CCK8 and colony formation test were used to detect their possible malignant transformation of biological behaviors.Results A high level of E-cadherin expression,high ability of proliferation and acquisition of anchorage-independent growth were found in CHO cells transfected by the E-cadherin plasmid.Conclusion An E-cadherin induced cancer model of ovarian epithelial cells was successfully constructed to provide a valuable tool to study the mechanisms of ovarian cancer.

关 键 词：E—cadherin 卵巢上皮细胞 恶变 细胞模型 

分 类 号：R737.31[医药卫生—肿瘤]