信号转导与转录因子3在宫颈癌组织中的表达及外周血表皮生长因子受体测定的意义  

The Significance of Expression of STAT3 in Cervical Cancer Tissue and EGFR Level in Peripheral Blood

作　　者：农文政[1] 马刚[2] 杜萍[1] 甘精华[1] 黄丽珍[1] 

机构地区：[1]广西壮族自治区民族医院,广西南宁530001 [2]广西壮族自治区人民医院,广西南宁530021

出　　处：《实用妇产科杂志》2010年第12期920-923,共4页Journal of Practical Obstetrics and Gynecology

摘　　要：目的:探讨信号转导与转录因子3(STAT3)及其上游基因表皮生长因子受体(EGFR)在宫颈癌中的表达及两者在宫颈癌发生发展中的作用和关系。方法:采用蛋白印记法(Western Blot)检测15例正常宫颈组织(健康对照组)、40例宫颈癌组织(宫颈癌组)中STAT3的表达及其磷酸化STAT3(p-STAT3)情况。双抗体夹心酶联免疫吸附法(ELISA)检测宫颈癌组患者治疗前后和健康对照组血清EGFR的含量。结果:①宫颈癌组织中STAT3表达水平和磷酸化水平明显高于健康对照组正常宫颈(P<0.05);②早期宫颈癌患者术前、术后血清中EGFR含量与健康对照组比较,差异无统计学意义(P=0.835;P=0.586;P=0.277);③中晚期宫颈癌患者术前、术后血清EGFR含量显著高于健康对照组(P=0.011;P=0.022;P=0.039)。治疗后EGFR含量稍有下降,治疗后14天与治疗前1天、治疗后7天比较明显下降,差异有统计学意义(P<0.05)。结论:STAT3高表达和激活能促进宫颈癌的发生、发展,晚期宫颈癌血清中EGFR测定可能成为宫颈癌的疗效评价及远期预后的指标。Objective：To investigate the expression of Signal Transducer and Activator of Transcription 3（STAT3） and its upstream gene EGFR and their roles in the pathogenesis of cervical cancer.Methods：Expression of STAT3 and its phosphorylated form were measured in 15 healthy cervix tissue and 40 cervical cancer tissue by Western blot. The level of EGFR in peripheral Blood of all the patients were measured by ELISA.Results：①The levels of unphosphorylated and phosphorylated STAT3 in cervical cancer were markedly higher than those in normal tissue （P〈0.05）.② The level of EGFR in peripheral blood before and after operation in early stages（stage Ⅰ and Ⅱ）were no difference significantly compared with healthy control（P=0.835;P=0.586;P=0.277）.③ The level of EGFR in peripheral blood before and after operation in advanced stages were markedly higher than those in healthy control group（P=0.011;P=0.022;P=0.039）.Conclusions：High expression and activation of STAT3 can promote the genesis of cervix cancer. EGFR level in peripheral blood of advanced cervical cancer may become a noble marker to evaluate prognosis and a monitor of curative effect.

关 键 词：信号转导与转录因子3 表皮生长因子受体 蛋白印记法 宫颈癌 双抗体夹心酶联免疫吸附法 

分 类 号：R737.33[医药卫生—肿瘤]