慢性成人牙周炎龈组织中Cyt-C、Caspase-9的表达和分布  

Expression and distribution of Cyt-C and Caspase-9 in gingival tissues with chronic periodontitis

作　　者：张菊梅[1] 王雪梅[1] 冯利[2] 余占海[1] 张轩[1] 

机构地区：[1]兰州大学口腔医学院,甘肃兰州730000 [2]兰州大学基础医学院,甘肃兰州730000

出　　处：《牙体牙髓牙周病学杂志》2010年第12期698-701,共4页Chinese Journal of Conservative Dentistry

摘　　要：目的:观察慢性牙周炎病人牙龈组织中凋亡细胞的形态、超微结构及其分布,并探讨其凋亡发生的途径。方法:利用组织病理学切片、透射电镜观察正常组织和牙周炎病人的牙龈组织中的凋亡细胞,并通过免疫组化SP法及计算机图像处理技术检测慢性牙周炎病人及牙龈健康者牙龈组织中Cyt-C、Caspase-9的分布及含量变化,并进行统计学分析。结果:慢性牙周炎病人龈组织较正常龈组织可观察明显的细胞凋亡现象,凋亡细胞以上皮中棘细胞层及基底层为主,Cyt-C、Caspase-9的表达也显著高于牙龈健康者(P﹤0.05)。结论:慢性牙周炎病人牙龈组织细胞发生凋亡,并依赖于线粒体途径。AIM： To observe whether apoptosis exists in gingival tissues of chronic periodontitis,and to explore the possible mechanisms of apoptosis.METHODS： Apoptotic cells in gingival tissues were observed by transmission electron microscopy.Expression and distribution of Casepase-9 and Cyt-C in gingival tissues of patients with chronic periodontitis and healthy individuals were examined by immunohistochemical method.RESULTS： Apoptotic cells were observed in gingival tissues of patients with chronic periodontitis,mainly in epithelium.The expression of Casepase-9 and Cyt-C in chronic periodontitis group was significantly higher compared to healthy group（P0.05）.CONCLUSION： Apoptosis exists in gingival tissues of chronic periodontitis,which is dependent on mitochondrial pathway.

关 键 词：凋亡细胞 慢性牙周炎 CYT-C CASPASE-9 免疫组织化学 

分 类 号：R780.2[医药卫生—口腔医学]