MHV-A59温病湿热小鼠模型的构建与相关指标分析  

Construction of MHV-A59 damp-heat mouse model and analysis of the relevant indices

作　　者：汤朝晖[1] 苏丽贤[1] 李华锋[2] 郭进强[1] 罗炳德[1] 林培政[2] 

机构地区：[1]南方医科大学公共卫生与热带医学学院,广东广州510515 [2]广州中医药大学温病教研室,广东广州510006

出　　处：《南方医科大学学报》2010年第11期2452-2454,共3页Journal of Southern Medical University

基　　金：国家自然科学基金-广东省联合基金(U0632009)~~

摘　　要：目的探讨MHV-A59病毒和湿热因素在炎症反应、水液代谢及免疫功能方面对MHV-A59小鼠肝炎湿热证模型构建的影响。方法将24只小鼠随机分为4组,根据上述2种因素分别设立健康对照组、病毒组、湿热组和模型组,用FCM法测定小鼠外周血CD4+%、CD8+%水平,血清IFN-γ、IL-4水平用ELISA法检测,小鼠肝脏NF-κB的表达和胃粘膜AQP4的表达用免疫组化法检测,用单因素方差分析进行统计学分析。结果病毒组与模型组小鼠肝脏NF-κB表达和CD4+/CD8+比值明显高于健康组和湿热组(P<0.01,P<0.05);而模型组和湿热组小鼠胃黏膜AQP4表达明显高于健康组(P<0.01);模型组小鼠IFN-γ/IL-4比值则较健康组升高(P<0.05)。结论 MHV-A59病毒是导致小鼠肝脏NF-κB表达增加及外周血CD4+/CD8+比值升高的主要因素,而湿热因素主要导致小鼠胃黏膜AQP4表达增加;病毒因素和湿热因素的协同作用可引起小鼠血清IFN-γ/IL-4比值的升高。利用MHV-A59病毒和湿热因素可以成功构建符合中医温病湿热证的小鼠模型。Objective To explore the impact of inflammation,water metabolism and immune function on the establishment of a mouse model of damp-heat syndrome with MHV-A59 infection.Methods Twenty-four mice were randomly divided into control group,virus group,damp-heat group and model group.The peripheral blood CD4＋ and CD8＋ lymphocytes were detected by flow cytometry,and the serum levels of IFN-γ and IL-4 were assayed by ELISA.The expressions of NF-κB and AQP4 in the liver and stomach were determined using immunohistochemistry.Results The expression of NF-κB and CD4＋/CD8＋ ratio in the virus and model groups were significantly higher than those in the damp-heat and control groups,while the expression of AQP4 was significantly higher in the model and damp-heat groups than in the other groups.Compared with the control group,the model group showed a significantly higher ratio of IFN-γ/IL-4.Conclusions MHV-A59 virus is the main cause of elevated NF-κB expression and CD4＋/CD8＋ ratio,while damp-heat syndrome is responsible for increased AQP4 expression,and their synergistic effect results in increased IFN-γ/IL-4 ratio.The mouse model established using MHV-A59 virus and the damp-heat factors can mimic damp-heat syndrome describedin traditional Chinese medicine theory.

关 键 词：MHV-A59病毒 湿热 模型小鼠 

分 类 号：R-332[医药卫生]