鼠舌癌发生过程中弹性纤维、胶原纤维的变化  

Changes of the elastic fibers and collagen fibers during the development and progression of experimentally induced tongue carcinoma in hamsters

作　　者：张鹏[1,2] 杜玉宾[1] 于淼[1] 殷翔[1] 吕岩红[1] 髙钟秀子 郑金华[1] 

机构地区：[1]哈尔滨医科大学解剖学教研室,黑龙江哈尔滨150081 [2]齐齐哈尔医学院解剖教研室,黑龙江齐齐哈尔161006

出　　处：《南方医科大学学报》2010年第12期2696-2698,共3页Journal of Southern Medical University

基　　金：教育部留学回国启动基金(教外司留(2007)1108);黑龙江省政府博士后启动基金(LRB-05-153)

摘　　要：目的观察金黄地鼠舌癌发生过程中弹性和胶原纤维的变化,探讨细胞外基质代谢紊乱与癌进展关系的本质。方法金黄地鼠舌癌存档蜡块,分为对照(6只)组和实验组(42只),实验组已有明确的病理分级(包括不典型增生、原位癌和早期浸润癌)。应用Masson和醛品红染色检测各组中弹性和胶原纤维的构筑和分布。结果在对照组AF染色阳性的纤细弹性纤维构成结缔组织芯的框架,在重度不典型增生和原位癌组弹性纤维呈现断裂和稀疏,在早期浸润癌组弹性纤维减少更为明显。对照组的胶原纤维表达低于实验组,在重度不典型增生和原位癌组胶原纤维显著增加,排列紊乱;在早期浸润癌组胶原纤维不仅增粗并沉积于粘膜固有层。结论癌的新生与鼠舌癌组织部位的胶原纤维过量沉积和弹性纤维明显减少的变化是引起舌癌生长和进展的重要诱因。Objective To investigate the relationship between the extracellular matrix （ECM） and neoplastic progression in hamster with tongue cancer. Methods Forty-eight specimens of hamster tongue cancer were divided into control group （n=6） and experimental group （n=42）. The pathological grade of the specimens was assessed （including 3 stages,namely atypical hyperplasia,carcinoma in situ and early invasive carcinoma）. The sections of the tongue were stained with Masson and aldehyde-fuchsin （AF） staining for microscopic observation of the elastic fiber and collagen fiber changes. Results Within the connective tissue cores （CTC） of the papillae in the control group was a framework of numerous and fine Gomrori＇s aldehyde fuchsin-positive elastic fibers. But in the stages of dysplasia and carcinoma in situ,these elastic fibers decreased and further diminished in the CTC in early invasive carcinoma. In dysplasia and carcinoma in situ stages,most of the elastic fibers collapsed with scattered elastic fibers,and the elastic fibers decreased significantly in early invasive carcinoma. The control group showed a significantly greater number of elastic fibers in the experimental group. The collagen fiber was obviously increased and irregularly arranged in dysplasia and carcinoma in situ stage; in early invasive carcinoma,the collagen fibers became thicker with deposition in the lamina propria. Conclusion An excessive deposition of collagen fiber and reduction of the elastic fibers is an important factor contributing to the development of tongue cancinoma in hamsters.

关 键 词：鼠舌癌 胶原纤维 弹性纤维 MASSON染色 醛品红染色 

分 类 号：R739.8[医药卫生—肿瘤]