裸大鼠人肝癌原位模型的建立及其生物学特性的初步观察  

Establishment and Biological Characteristics of Orthotopic Transplantion Tumor Model of Hepatocellar Carcinoma Cell line in Nude Rat

作　　者：沈艳[1] 彭秀华[1] 徐春华[1] 周文江[1,2] 

机构地区：[1]上海市公共卫生临床中心,上海201508 [2]复旦大学实验动物科学部,上海200032

出　　处：《实验动物与比较医学》2010年第6期428-431,共4页Laboratory Animal and Comparative Medicine

摘　　要：目的 探讨人肝癌原位移植后肿瘤在裸大鼠（rnu/rnu）生长和转移等生物学特性.方法 Huh-7细胞株接种于裸小鼠皮下成瘤组织块移植于裸大鼠皮下成瘤,之后将裸大鼠皮下肿瘤移植至其肝脏以建立原位移植模型,观察所建立模型原位成瘤率、移植瘤生长、侵袭和转移情况,同时进行病理组织学、超微结构、细胞增殖周期和异倍体的观察.结果 原位移植瘤模型裸大鼠成瘤率高达90.0%,其肺转移率形成率为50.0%.结论 应用裸大鼠建立肝癌原位移植模型,为探讨肝癌转移的生物学机制和抗转移治疗提供了理想的实验动物模型.Objective To study the growth and metastases of the tumors after orthotopic transplantation with hepatocellar carcinoma cell line （Huh-7） in nude rat. Methods Tissue from the tumor inoculated subcutaneously in nude mice with Huh-7 cell line was transferred to the nude rat beneath the skin. Subcutaneous and orthotopic implant models simulating hepatocellar carcinoma was established by suturing histologically intact human tumor tissue on the liver of nude rat. Subsequently,the rate of the orthotopic tumor and their biological properties of tumor growth and metastasis were observed. Tumorgenecity, invasion, metastasis and morphological characteristics of the implanted tumors were studied by light microscopy, electron microscopy and flow cytometry. Results In orthotopic trans- plant model of nude rat, tumor formation rate was 90.0% and metastasis rate of liver was 50.0%. Conclusions The nude rat is more suitable for orthotopic transplant models for hepatocellar carcinoma, and should be valuable for research on mechanism and new therapies for metastasis.

关 键 词：裸大鼠(rnu/rnu) 皮下移植:原位移植 肝癌 

分 类 号：R735.7[医药卫生—肿瘤] R-332[医药卫生—临床医学]