高脂饮食喂养大鼠脂肪组织GRP78mRNA的表达及意义  

Expression of GRP78 mRNA in Obese Tissue of Rats Fed with High Fat Diet

作　　者：刘佳[1] 都健[1] 裴丽娜[1] 罗红飞[1] 郭宁宁[1] 林志楠[1] 张克莹[1] 

机构地区：[1]中国医科大学附属第一医院内分泌科,沈阳110001

出　　处：《实验动物与比较医学》2010年第6期436-439,444,共5页Laboratory Animal and Comparative Medicine

基　　金：辽宁省科技厅资助项目,项目编号为2009225029

摘　　要：目的 通过观察高脂喂养的胰岛素抵抗大鼠内脏脂肪组织中GRP78mRNA的表达,探讨其在胰岛素抵抗发病机制中的意义.方法 30只雄性Wistar大鼠随机分为正常饮食组（NC组）和高脂饮食组（HF组）,每组15只.喂养10周后,应用高胰岛素-正常血糖钳夹实验技术评估胰岛素抵抗模型的建立.采用实时荧光定量PCR（Real time PCR）法检测脂肪组织GRP78mRNA的表达.同时检测血清胆固醇（TC）、甘油三酯（TG）、测定血糖（BG）、胰岛素（INS）,测定各组大鼠内脏脂肪的重量与体重的比值.结果 （1）喂养10周后HF组的葡萄搪输注率（GIR）明显低于NC组（P〈0.01）,HF组胰岛素明显高于NC组（P〈0.05）;（2）HF组大鼠内脏脂肪组织中GRP78mRNA的表达明显增高,与NC组比较差异有统计学意义（P〈0.01）;（3）10周末,HF组大鼠甘油三酯（TG）、胆固醇（TC）、及内脏脂肪相对含量均明显升高（P〈0.01）.结论 高脂喂养10周大鼠胰岛素抵抗模型建立成功,高脂饮食可诱导内脏脂肪组织GRP78mRNA表达增强.Objective To observe the expression of GRP78 mRNA in visceral obese of rats fed with high fat diet in order to explore its effects on insulin resistance. Methods Thirty Wistar rats were randomly divided into two groups： normal chow group （NC） was fed with free normal chow for 10 weeks; high fat group （HF） was fed with high fat diet for 10 weeks. The expression of GRP78 mRNA was detected by real time PCR. The total cholesterol ,triglyceride ,the rate of visceral fat （epididymal fat） vs. body weight and fasting insulin were also detected.Results （1） Glucose infusion rate （GIR） in HF group was obviously lower than that in NC group （P〈0.01）. Rats in HF group had an elevation of fasting insulin compared with NC group （P〈0.05）. （2） The expression of GRP78 mRNA was significantly higher in HF group than that in NC group （P〈0.01）. （3） Rats in HF group had an obviously elevation of fasting insulin ,total cholesterol and triglyceride compared with NC group .Conclusion After 10 weeks fed with high fat diet, the insulin resistant animal models was established. High fat diet can induce the expression of GRP78 mRNA in visceral obese tissue.

关 键 词：内质网应激 胰岛素抵抗 GRP78 脂肪组织 

分 类 号：R-332[医药卫生] R587.1