流动注射褪色法测定食品中的亚硫酸盐  

Determination of Food Sulfite by Flow-injection Analysis with Color-fading Spectrometric Method

作　　者：曹凤梅 张新申[1] 赵欢欢[2] 杨育杰[2] 孙静[2] 李辉[2] 

机构地区：[1]四川大学制革清洁技术国家工程实验室,四川成都610065 [2]四川大学建筑与环境学院,四川成都610065

出　　处：《皮革科学与工程》2011年第2期56-59,共4页Leather Science and Engineering

摘　　要：建立了一种用流动注射测定亚硫酸盐的新方法。在pH=6的缓冲介质中亚硫酸盐使孔雀石绿溶液褪色,褪色程度与亚硫酸盐浓度线性相关。测定浓度范围是2×10-4～2.6×10-2 g/L,线性方程为y=13.982x-0.3703,其中R2=0.9991。测定用最大吸收波长为615 nm。此法与国标比较误差在±5%范围内,方法简单,方便,应用在食品测定中结果令人满意。Established one kind of new method to determine sulfite in food by flow-injection analysis.The paper shows that in a solution of pH=6.0 phosphate buffer medium,sulfite can cause a color-fading of the malachite green which has the maximum absorption wavelength at 615 nm.The determination of sulfite is carried out by measuring the intensity of color-fading,and they are linearly dependent.The measuring range is from 2×10-4 g/L to 2.6×10-2 g/L with a correlation coefficient of 0.9991 and the regression equation is y=13.982x-0.3703 By Comparing with National Standard Method of China,the error is within ±5% which meets the requirements.The method of this paper is simple,convenient,and to determine the sulfite in food which can have satisfactory results.

关 键 词：流动注射 亚硫酸盐 孔雀石绿 褪色法 

分 类 号：O657.3[理学—分析化学]