解除颈动脉狭窄对慢性低灌注大鼠氧化损伤的影响  

Influence of relieving carotid stenosis on oxidative damage in chronic cerebral hypoperfusion rats

作　　者：冉昌丽[1] 李耀彩[1] 张其梅[1] 张强[1] 

机构地区：[1]三峡大学第一临床医学院宦昌市中心人民医院神经内科,宜昌443003

出　　处：《中华老年心脑血管病杂志》2011年第4期370-371,共2页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

摘　　要：目的探讨解除颈动脉狭窄对慢性低灌注大鼠认知功能损伤的影响及其可能机制。方法选择雄性SD大鼠30只,将制模成功大鼠12只随机分为狭窄组和狭窄解除组,每组6只。另选6只大鼠为假手术组。采用分光光度法测定超氧化物歧化酶(SOD)活性和丙二醛的含量。结果与假手术组比较,狭窄组大鼠SOD活性明显下降,丙二醛的含量明显升高,差异有统计学意义(P<0.01);与狭窄组比较,狭窄解除组大鼠SOD活性明显升高,丙二醛含量明显下降,差异有统计学意义(P<0.01)。结论氧化损伤参与了重度颈动脉狭窄所致认知功能障碍的作用机制;且解除颈动脉狭窄改善认知功能障碍的作用机制可能与改善脑血流、抗氧化作用有关。Objective To investigate the influence of relieving carotid stenosis on cognitive function of rats with chronic cerebral hypoperfusion injury and its possible mechanism. Methods Thirty male SD rats were selected, 12 model rats were randomly divided into stenosis group and stenosis- relieving group of 6 rats each. Other 6 rats served as sham-operated group. Speetrophotometry was used to determine superoxide dismutase（SOD） activity and malondialdehyde（MDA） content. Results Compared with the sham group,the SOD activity was significantly decreased and MDA content significantly increased in stenosis group,the differences were statistically significant （P 〈0.01）. Compared with stenosis group, the activity of SOD significantly increased and MDA con- centration significantly decreased in stenosis-relieving group （P 〈 0.01）. Conclusion Oxidative damage caused by severe carotid artery stenosis participates in the mechanism of cognitive impair- ment ;and the mechanism of improving cognitive impairment by relieving stenosis may be associat- ed with improving cerebral blood flow and antioxidative effects.

关 键 词：认知障碍 脑缺血 颈动脉狭窄 超氧化物歧化酶 丙二醛 危险因素 

分 类 号：R749.13[医药卫生—神经病学与精神病学]