难治性淋巴瘤患者外周血T淋巴细胞亚群与自然杀伤细胞活性的检测及其临床意义  

Detection and clinical significance of T cell subgroups and NK cell activity in refractory lymphoma patients

作　　者：武晓博[1] 张巧花[2] 侯淑玲[2] 孙俊宁[3] 贾莉[3] 苏文[3] 郭润莲[2] 

机构地区：[1]山西医科大学研究生院,太原030001 [2]山西省肿瘤医院血液内科 [3]山西省肿瘤研究所免疫室

出　　处：《白血病．淋巴瘤》2011年第3期162-164,共3页Journal of Leukemia & Lymphoma

摘　　要：目的探讨外周血T淋巴细胞亚群及自然杀伤（NK）细胞活性水平与难治性淋巴瘤的相关性。方法采用流式细胞术（FCM）检测60例初治淋巴瘤患者化疗前外周血T淋巴细胞亚群水平与NK细胞的活性，化疗后随访分为难治组30例、有效组30例，以20名健康者为健康对照组。结果淋巴瘤患者组化疗前外周血CD4^＋、CD4^＋／CD8^＋、NK细胞数比健康对照组低（30．17±8．63与46．52±1．39，t=12．218，P〈0．05；0．86±0．45与1．64±0．05，t=11．225，P〈0．05；12．39±7．08与19．29±0．84，t=6．365，P〈0．05），CD3^＋、CD8^＋细胞数比健康对照组高（76．14±10．71与70．48±1．44，t=-3．439，P〈0．05；40．28±14．03与28．35±0．73，t=-5．625，P〈0．05）。难治组化疗前外周血CD4^＋、CD4^＋／CD^8＋、NK细胞数比有效组低（27．70±7．81与33．13±8．82，t=2．163，P=0．036；0．67±0．27与1．10±0．52，t=3．272，P=0．003；9．87±6．60与15．40±6．58，t=2．771，P=0．008），而CD3^＋、CD8^＋细胞数比有效组高（79．67±8．18与71．91±12．00，t=-2．540，P=0．015；44．70±13．99与34．98±12．41，t=-2．416，P=0．020）。结论淋巴瘤初治患者化疗前外周血T淋巴细胞亚群水平及NK细胞活性的检测，对判断、预测容易转归为难治的患者可能有一定的参考价值。Objective To investigate the correlation of T cell subgroups and natural killer （NK）＇celi s activity level of peripheral blood of the patients with refractory lymphoma. Methods Flow cytometry was applied to detect T cell subgroups＇ level and NK cell＇s activity of peripheral blood in 60 early cure lymphoma patients with chemotherapy before and 20 normal controls , after chemotherapy follow-up they were divided into 30 cases of difficult cure group and 30 cases of effective group. Results Compared with the normal controls, CD4^＋, CD4^＋/CD8^＋ and NK cell in lymphoma patients with chemotherapy before decreased （30.17±8.63 vs 46.52±1.39, t =12.218, P 〈0.05; 0.86±0.45 vs 1.64±0.05, t =11.225, P 〈0.05; 12.39±7.08 vs 19.29±0.84, t =6.365, P〈0.05）, while CD3^＋ and CD8^＋ cell increased （76.14±10.71 vs 70.48±1.44, t =-3.439, P 〈0.05; 40.28±14.03 vs 28.35-±0.73, t =-5.625, P 〈0.05）. Compared with effective group, CD4^＋, CD4^＋/CD8^＋ and NK cell in difficult response group with chemotherapy before decreased （27.70±7.81 vs 33.13±8.82, t =2.163, P =0.036; 0.67±0.27 vs 1.10±0.52, t =3.272, P =0.003; 9.87±6.60 vs 15.40±6.58, t =2.771, P =0.008）, while CD3^＋ and CD8^＋ cell increased （79.67±8.18 vs 71.91±12.00, t =-2.540, P =0.015; 44.70±13.99 vs 34.98±12.41, t =-2.416, P =0.020）. Conclusion The detection of T cell subgroups＂ level and NK cell＂ s activity in early lymphoma patients before chemotherapy may play a role to diagnose and predict the outcome of refractory lymphoma patients.

关 键 词：难治性淋巴瘤 T淋巴细胞亚群 杀伤细胞 天然 

分 类 号：R733.1[医药卫生—肿瘤]